7SC2

CRYSTAL STRUCTURE OF THE N-DOMAIN OF CARDIAC MUSCLE TROPONIN C TETHERED TO THE SWITCH REGION OF CARDIAC MUSCLE TROPONIN I (TETRAGONAL FORM)

7SC2 の概要

• エントリーDOI: 10.2210/pdb7sc2/pdb
• 分子名称: Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle chimera, CALCIUM ION (3 entities in total)
• 機能のキーワード: calcium sensitizer, contraction regulation, contractile protein, cardiac troponin, muscle regulation, calcium-binding, ef-hand
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13340.27

構造登録者

Sack, J.S. (登録日: 2021-09-27, 公開日: 2021-12-01, 最終更新日: 2024-05-22)

主引用文献

Yan, C.,Sack, J.S.
X-ray structure of a human cardiac muscle troponin C/troponin I chimera in two crystal forms.
Acta Crystallogr.,Sect.F, 78:17-24, 2022

PubMed Abstract: The X-ray crystal structure of a human cardiac muscle troponin C/troponin I chimera has been determined in two different crystal forms and shows a conformation of the complex that differs from that previously observed by NMR. The chimera consists of the N-terminal domain of troponin C (cTnC; residues 1-80) fused to the switch region of troponin I (cTnI; residues 138-162). In both crystal forms, the cTnI residues form a six-turn α-helix that lays across the hydrophobic groove of an adjacent cTnC molecule in the crystal structure. In contrast to previous models, the cTnI helix runs in a parallel direction relative to the cTnC groove and completely blocks the calcium desensitizer binding site of the cTnC-cTnI interface.

PubMed: 34981771
DOI: 10.1107/S2053230X21012395

実験手法

X-RAY DIFFRACTION (1.814 Å)