7RTM

Cryo-EM Structure of the Sodium-driven Chloride/Bicarbonate Exchanger NDCBE (SLC4A8)

7RTM の概要

• エントリーDOI: 10.2210/pdb7rtm/pdb
• EMDBエントリー: 24683
• 分子名称: Electroneutral sodium bicarbonate exchanger 1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: ndcbe, sodium-driven chloride/bicarbonate exchanger, cryo-em, membrane protein
• 由来する生物種: Oryctolagus cuniculus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 130471.79

構造登録者

Wang, W.G.,Tsirulnikov, K.,Zhekova, H.,Kayik, G.,Muhammad-Khan, H.,Azimov, R.,Abuladze, N.,Kao, L.,Newman, D.,Noskov, S.Y.,Zhou, Z.H.,Pushkin, A.,Kurtz, I. (登録日: 2021-08-13, 公開日: 2021-09-29, 最終更新日: 2021-10-06)

主引用文献

Wang, W.,Tsirulnikov, K.,Zhekova, H.R.,Kayik, G.,Khan, H.M.,Azimov, R.,Abuladze, N.,Kao, L.,Newman, D.,Noskov, S.Y.,Zhou, Z.H.,Pushkin, A.,Kurtz, I.
Cryo-EM structure of the sodium-driven chloride/bicarbonate exchanger NDCBE.
Nat Commun, 12:5690-5690, 2021

PubMed Abstract: SLC4 transporters play significant roles in pH regulation and cellular sodium transport. The previously solved structures of the outward facing (OF) conformation for AE1 (SLC4A1) and NBCe1 (SLC4A4) transporters revealed an identical overall fold despite their different transport modes (chloride/bicarbonate exchange versus sodium-carbonate cotransport). However, the exact mechanism determining the different transport modes in the SLC4 family remains unknown. In this work, we report the cryo-EM 3.4 Å structure of the OF conformation of NDCBE (SLC4A8), which shares transport properties with both AE1 and NBCe1 by mediating the electroneutral exchange of sodium-carbonate with chloride. This structure features a fully resolved extracellular loop 3 and well-defined densities corresponding to sodium and carbonate ions in the tentative substrate binding pocket. Further, we combine computational modeling with functional studies to unravel the molecular determinants involved in NDCBE and SLC4 transport.

PubMed: 34584093
DOI: 10.1038/s41467-021-25998-2

実験手法

ELECTRON MICROSCOPY (3.4 Å)