7RLM

Crystal Structure of Potato Leafroll Virus (PLRV) Coat Protein

7RLM の概要

• エントリーDOI: 10.2210/pdb7rlm/pdb
• 分子名称: Coat protein, BROMIDE ION (3 entities in total)
• 機能のキーワード: plrv, polerovirus, icosahedral virus, quasi-equivalence, viral assembly, capsid, plant virus, viral protein
• 由来する生物種: Potato leafroll virus (PLrV)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 93496.39

構造登録者

Adams, M.C.,Chappie, J.S.,Schiltz, C.J. (登録日: 2021-07-25, 公開日: 2022-01-19, 最終更新日: 2023-10-18)

主引用文献

Adams, M.C.,Schiltz, C.J.,Heck, M.L.,Chappie, J.S.
Crystal structure of the potato leafroll virus coat protein and implications for viral assembly.
J.Struct.Biol., 214:107811-107811, 2021

PubMed Abstract: Luteoviruses, poleroviruses, and enamoviruses are insect-transmitted, agricultural pathogens that infect a wide array of plants, including staple food crops. Previous cryo-electron microscopy studies of virus-like particles show that luteovirid viral capsids are built from a structural coat protein that organizes with T = 3 icosahedral symmetry. Here, we present the crystal structure of a truncated version of the coat protein monomer from potato leafroll virus at 1.80-Å resolution. In the crystal lattice, monomers pack into flat sheets that preserve the two-fold and three-fold axes of icosahedral symmetry and show minimal structural deviations when compared to the full-length subunits of the assembled virus-like particle. These observations have important implications in viral assembly and maturation and suggest that the CP N-terminus and its interactions with RNA play an important role in generating capsid curvature.

PubMed: 34813955
DOI: 10.1016/j.jsb.2021.107811

実験手法

X-RAY DIFFRACTION (1.8 Å)