7RJC

Complex III2 from Candida albicans, inhibitor free, Rieske head domain in intermediate position

7RJC の概要

• エントリーDOI: 10.2210/pdb7rjc/pdb
• EMDBエントリー: 24482 24483 24484 24485 24486
• 分子名称: Ubiquinol--cytochrome-c reductase subunit, PROTOPORPHYRIN IX CONTAINING FE, UBIQUINONE-10, ... (13 entities in total)
• 機能のキーワード: candida albicans, mitochondrial complex iii2, indazole-derivative inhibitor, rieske head domain, membrane protein
• 由来する生物種: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 259965.38

構造登録者

Di Trani, J.M.,Rubinstein, J.L. (登録日: 2021-07-20, 公開日: 2021-09-15, 最終更新日: 2024-12-25)

主引用文献

Di Trani, J.M.,Liu, Z.,Whitesell, L.,Brzezinski, P.,Cowen, L.E.,Rubinstein, J.L.
Rieske head domain dynamics and indazole-derivative inhibition of Candida albicans complex III.
Structure, 30:129-, 2022

PubMed Abstract: Electron transfer between respiratory complexes drives transmembrane proton translocation, which powers ATP synthesis and membrane transport. The homodimeric respiratory complex III (CIII) oxidizes ubiquinol to ubiquinone, transferring electrons to cytochrome c and translocating protons through a mechanism known as the Q cycle. The Q cycle involves ubiquinol oxidation and ubiquinone reduction at two different sites within each CIII monomer, as well as movement of the head domain of the Rieske subunit. We determined structures of Candida albicans CIII by cryoelectron microscopy (cryo-EM), revealing endogenous ubiquinone and visualizing the continuum of Rieske head domain conformations. Analysis of these conformations does not indicate cooperativity in the Rieske head domain position or ligand binding in the two CIIIs of the CIII dimer. Cryo-EM with the indazole derivative Inz-5, which inhibits fungal CIII and is fungicidal when administered with fungistatic azole drugs, showed that Inz-5 inhibition alters the equilibrium of Rieske head domain positions.

PubMed: 34525326
DOI: 10.1016/j.str.2021.08.006

実験手法

ELECTRON MICROSCOPY (3.3 Å)