7RBE

Human DNA polymerase beta crosslinked binary complex - A

7RBE の概要

• エントリーDOI: 10.2210/pdb7rbe/pdb
• 分子名称: DNA polymerase beta, DNA (5'-D(*GP*TP*CP*GP*G)-3'), DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*C)-3'), ... (8 entities in total)
• 機能のキーワード: protein-dna crosslink, polymerase, lyase, drp, transferase, transferase-dna complex, transferase/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 49309.05

構造登録者

Reed, A.J.,Kumar, A. (登録日: 2021-07-06, 公開日: 2022-03-09, 最終更新日: 2024-10-30)

主引用文献

Kumar, A.,Reed, A.J.,Zahurancik, W.J.,Daskalova, S.M.,Hecht, S.M.,Suo, Z.
Interlocking activities of DNA polymerase beta in the base excision repair pathway.
Proc.Natl.Acad.Sci.USA, 119:e2118940119-e2118940119, 2022

PubMed Abstract: SignificanceBase excision repair (BER) is one of the major DNA repair pathways used to fix a myriad of cellular DNA lesions. The enzymes involved in BER, including DNA polymerase β (Polβ), have been identified and characterized, but how they act together to efficiently perform BER has not been fully understood. Through gel electrophoresis, mass spectrometry, and kinetic analysis, we discovered that the two enzymatic activities of Polβ can be interlocked, rather than functioning independently from each other, when processing DNA intermediates formed in BER. The finding prompted us to hypothesize a modified BER pathway. Through conventional and time-resolved X-ray crystallography, we solved 11 high-resolution crystal structures of cross-linked Polβ complexes and proposed a detailed chemical mechanism for Polβ's 5'-deoxyribose-5-phosphate lyase activity.

PubMed: 35238634
DOI: 10.1073/pnas.2118940119

実験手法

X-RAY DIFFRACTION (1.89 Å)