7RAI
Cryo-EM structure of M4008_N1 Fab in complex with BG505 DS-SOSIP.664 Env trimer
Summary for 7RAI
| Entry DOI | 10.2210/pdb7rai/pdb |
| EMDB information | 24362 |
| Descriptor | Envelope glycoprotein gp160, HIV-1 Envelope Glycoprotein BG505 SOSIP.664 gp41, M4008_N1 Fab heavy chain, ... (9 entities in total) |
| Functional Keywords | hiv, envelope, bnab, complex, immune system |
| Biological source | Human immunodeficiency virus 1 More |
| Total number of polymer chains | 12 |
| Total formula weight | 392522.95 |
| Authors | Chan, K.-W.,Kong, X.P. (deposition date: 2021-07-01, release date: 2021-11-17, Last modification date: 2024-11-06) |
| Primary citation | Chan, K.W.,Luo, C.C.,Lu, H.,Wu, X.,Kong, X.P. A site of vulnerability at V3 crown defined by HIV-1 bNAb M4008_N1. Nat Commun, 12:6464-6464, 2021 Cited by PubMed Abstract: Identification of vulnerable sites defined by broadly neutralizing antibodies (bNAbs) on HIV-1 envelope (Env) is crucial for vaccine design, and we present here a vulnerable site defined by bNAb M4008_N1, which neutralizes about 40% of a tier-2 virus panel. A 3.2 Å resolution cryo-EM structure of M4008_N1 in complex with BG505 DS-SOSIP reveals a large, shallow protein epitope surface centered at the V3 crown of gp120 and surrounded by key glycans. M4008_N1 interacts with gp120 primarily through its hammerhead CDR H3 to form a β-sheet interaction with the V3 crown hairpin. This makes M4008_N1 compatible with the closed conformation of the prefusion Env trimer, and thus distinct from other known V3 crown mAbs. This mode of bNAb approaching the immunogenic V3 crown in the native Env trimer suggests a strategy for immunogen design targeting this site of vulnerability. PubMed: 34753944DOI: 10.1038/s41467-021-26846-z PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.24 Å) |
Structure validation
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