7RAI

Cryo-EM structure of M4008_N1 Fab in complex with BG505 DS-SOSIP.664 Env trimer

7RAI の概要

• エントリーDOI: 10.2210/pdb7rai/pdb
• EMDBエントリー: 24362
• 分子名称: Envelope glycoprotein gp160, HIV-1 Envelope Glycoprotein BG505 SOSIP.664 gp41, M4008_N1 Fab heavy chain, ... (9 entities in total)
• 機能のキーワード: hiv, envelope, bnab, complex, immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 392522.95

構造登録者

Chan, K.-W.,Kong, X.P. (登録日: 2021-07-01, 公開日: 2021-11-17, 最終更新日: 2024-11-06)

主引用文献

Chan, K.W.,Luo, C.C.,Lu, H.,Wu, X.,Kong, X.P.
A site of vulnerability at V3 crown defined by HIV-1 bNAb M4008_N1.
Nat Commun, 12:6464-6464, 2021

PubMed Abstract: Identification of vulnerable sites defined by broadly neutralizing antibodies (bNAbs) on HIV-1 envelope (Env) is crucial for vaccine design, and we present here a vulnerable site defined by bNAb M4008_N1, which neutralizes about 40% of a tier-2 virus panel. A 3.2 Å resolution cryo-EM structure of M4008_N1 in complex with BG505 DS-SOSIP reveals a large, shallow protein epitope surface centered at the V3 crown of gp120 and surrounded by key glycans. M4008_N1 interacts with gp120 primarily through its hammerhead CDR H3 to form a β-sheet interaction with the V3 crown hairpin. This makes M4008_N1 compatible with the closed conformation of the prefusion Env trimer, and thus distinct from other known V3 crown mAbs. This mode of bNAb approaching the immunogenic V3 crown in the native Env trimer suggests a strategy for immunogen design targeting this site of vulnerability.

PubMed: 34753944
DOI: 10.1038/s41467-021-26846-z

実験手法

ELECTRON MICROSCOPY (3.24 Å)