7R9G

Catalytically inactive yeast Pseudouridine Synthase, PUS1, bound to RNA

7R9G の概要

• エントリーDOI: 10.2210/pdb7r9g/pdb
• 分子名称: tRNA pseudouridine synthase 1, RNA (5'-R(*AP*AP*AP*UP*CP*GP*GP*GP*AP*UP*UP*CP*CP*GP*GP*AP*UP*A)-3'), SULFATE ION, ... (5 entities in total)
• 機能のキーワード: pseudouridine, pus, pus1, rna, synthase, mrna, yeast, pseudo uracil, pseudouracil, uracil, saccharomyces cerevisiae, inactive, catalytically inactive, dead, pseudouridine synthase, rna binding protein, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68125.58

構造登録者

Doyle, L.A.,Stoddard, B.L. (登録日: 2021-06-29, 公開日: 2021-12-15, 最終更新日: 2023-11-15)

主引用文献

Grunberg, S.,Doyle, L.A.,Wolf, E.J.,Dai, N.,Correa Jr., I.R.,Yigit, E.,Stoddard, B.L.
The structural basis of mRNA recognition and binding by yeast pseudouridine synthase PUS1.
Plos One, 18:e0291267-e0291267, 2023

PubMed Abstract: The chemical modification of RNA bases represents a ubiquitous activity that spans all domains of life. Pseudouridylation is the most common RNA modification and is observed within tRNA, rRNA, ncRNA and mRNAs. Pseudouridine synthase or 'PUS' enzymes include those that rely on guide RNA molecules and others that function as 'stand-alone' enzymes. Among the latter, several have been shown to modify mRNA transcripts. Although recent studies have defined the structural requirements for RNA to act as a PUS target, the mechanisms by which PUS1 recognizes these target sequences in mRNA are not well understood. Here we describe the crystal structure of yeast PUS1 bound to an RNA target that we identified as being a hot spot for PUS1-interaction within a model mRNA at 2.4 Å resolution. The enzyme recognizes and binds both strands in a helical RNA duplex, and thus guides the RNA containing the target uridine to the active site for subsequent modification of the transcript. The study also allows us to show the divergence of related PUS1 enzymes and their corresponding RNA target specificities, and to speculate on the basis by which PUS1 binds and modifies mRNA or tRNA substrates.

PubMed: 37939088
DOI: 10.1371/journal.pone.0291267

実験手法

X-RAY DIFFRACTION (2.4 Å)