7R96

Self-Assembled 3D DNA Hexagonal Tensegrity Triangle

7R96 の概要

• エントリーDOI: 10.2210/pdb7r96/pdb
• 分子名称: DNA (5'-D(*AP*GP*GP*CP*AP*GP*CP*CP*TP*GP*TP*AP*CP*GP*GP*AP*CP*AP*TP*CP*A)-3'), DNA (5'-D(P*CP*CP*GP*TP*AP*CP*A)-3'), DNA (5'-D(P*GP*GP*CP*TP*GP*C)-3'), ... (4 entities in total)
• 機能のキーワード: tensegrity triangle, synthetic construct, self-assembly, dna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 12797.42

構造登録者

Lu, B.,Vecchioni, S.,Ohayon, Y.P.,Sha, R.,Mao, C.,Seeman, N.C. (登録日: 2021-06-28, 公開日: 2021-10-20, 最終更新日: 2023-10-18)

主引用文献

Lu, B.,Vecchioni, S.,Ohayon, Y.P.,Sha, R.,Woloszyn, K.,Yang, B.,Mao, C.,Seeman, N.C.
3D Hexagonal Arrangement of DNA Tensegrity Triangles.
Acs Nano, 15:16788-16793, 2021

PubMed Abstract: The tensegrity triangle motif utilizes Watson-Crick sticky end cohesion to self-assemble into a rhombohedral crystal lattice using complementary 5'-GA and 5'-TC sticky ends. Here, we report that using noncanonical 5'-AG and 5'-TC sticky ends in otherwise isomorphic tensegrity triangles results in crystal self-assembly in the 6 hexagonal space group as revealed by X-ray crystallography. In this structure, the DNA double helices bend at the crossover positions, a feature that was not observed in the original design. Instead of propagating linearly, the tilt between base pairs of each right-handed helix results in a left-handed superstructure along the screw axis, forming a microtubule-like structure composed of three double helices with an unbroken channel at the center. This hexagonal lattice has a cavity diameter of 11 nm and a unit cell volume of 886 000 Å-far larger than the rhombohedral counterpart (5 nm, 330 000 Å).

PubMed: 34609128
DOI: 10.1021/acsnano.1c06963

実験手法

X-RAY DIFFRACTION (5.68 Å)