7R8H

Crystal structure of Pseudooceanicola lipolyticus Argonaute bound to 5' p guide DNA

7R8H の概要

• エントリーDOI: 10.2210/pdb7r8h/pdb
• 分子名称: Argonaute, DNA (5'-D(*TP*TP*AP*CP*TP*GP*CP*AP*CP*AP*GP*GP*TP*GP*AP*CP*GP*A)-3') (2 entities in total)
• 機能のキーワード: argonaute, ago, dna guide, rna cleavage, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Pseudooceanicola lipolyticus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 93455.34

構造登録者

Shin, Y.,Murakami, K.S. (登録日: 2021-06-26, 公開日: 2022-07-06, 最終更新日: 2024-05-22)

主引用文献

Lisitskaya, L.,Shin, Y.,Agapov, A.,Olina, A.,Kropocheva, E.,Ryazansky, S.,Aravin, A.A.,Esyunina, D.,Murakami, K.S.,Kulbachinskiy, A.
Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity.
Nat Commun, 13:4624-4624, 2022

PubMed Abstract: Argonaute proteins are programmable nucleases that have defense and regulatory functions in both eukaryotes and prokaryotes. All known prokaryotic Argonautes (pAgos) characterized so far act on DNA targets. Here, we describe a new class of pAgos that uniquely use DNA guides to process RNA targets. The biochemical and structural analysis of Pseudooceanicola lipolyticus pAgo (PliAgo) reveals an unusual organization of the guide binding pocket that does not rely on divalent cations and the canonical set of contacts for 5'-end interactions. Unconventional interactions of PliAgo with the 5'-phosphate of guide DNA define its new position within pAgo and shift the site of target RNA cleavage in comparison with known Argonautes. The specificity for RNA over DNA is defined by ribonucleotide residues at the cleavage site. The analysed pAgos sense mismatches and modifications in the RNA target. The results broaden our understanding of prokaryotic defense systems and extend the spectrum of programmable nucleases with potential use in RNA technology.

PubMed: 35941106
DOI: 10.1038/s41467-022-32079-5

実験手法

X-RAY DIFFRACTION (2.54 Å)