7R55

B-trefoil lectin from Salpingoeca rosetta in complex with Gb3

これはPDB形式変換不可エントリーです。

7R55 の概要

• エントリーDOI: 10.2210/pdb7r55/pdb
• 分子名称: Sarol-1, alpha-D-galactopyranose-(1-4)-beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: lectin, gb3, b-trefoil, pore forming lectin, sugar binding protein
• 由来する生物種: Salpingoeca rosetta
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 75115.39

構造登録者

Notova, S.,Varrot, A. (登録日: 2022-02-10, 公開日: 2022-09-14, 最終更新日: 2024-01-31)

主引用文献

Notova, S.,Bonnardel, F.,Rosato, F.,Siukstaite, L.,Schwaiger, J.,Lim, J.H.,Bovin, N.,Varrot, A.,Ogawa, Y.,Romer, W.,Lisacek, F.,Imberty, A.
The choanoflagellate pore-forming lectin SaroL-1 punches holes in cancer cells by targeting the tumor-related glycosphingolipid Gb3.
Commun Biol, 5:954-954, 2022

PubMed Abstract: Choanoflagellates are primitive protozoa used as models for animal evolution. They express a large variety of multi-domain proteins contributing to adhesion and cell communication, thereby providing a rich repertoire of molecules for biotechnology. Adhesion often involves proteins adopting a β-trefoil fold with carbohydrate-binding properties therefore classified as lectins. Sequence database screening with a dedicated method resulted in TrefLec, a database of 44714 β-trefoil candidate lectins across 4497 species. TrefLec was searched for original domain combinations, which led to single out SaroL-1 in the choanoflagellate Salpingoeca rosetta, that contains both β-trefoil and aerolysin-like pore-forming domains. Recombinant SaroL-1 is shown to bind galactose and derivatives, with a stronger affinity for cancer-related α-galactosylated epitopes such as the glycosphingolipid Gb3, when embedded in giant unilamellar vesicles or cell membranes. Crystal structures of complexes with Gb3 trisaccharide and GalNAc provided the basis for building a model of the oligomeric pore. Finally, recognition of the αGal epitope on glycolipids required for hemolysis of rabbit erythrocytes suggests that toxicity on cancer cells is achieved through carbohydrate-dependent pore-formation.

PubMed: 36097056
DOI: 10.1038/s42003-022-03869-w

実験手法

X-RAY DIFFRACTION (1.84 Å)