7R4X

Cryo-EM reconstruction of the human 40S ribosomal subunit - Full map

7R4X の概要

• エントリーDOI: 10.2210/pdb7r4x/pdb
• EMDBエントリー: 14317 14318 14319
• 分子名称: 60S ribosomal protein L41, 40S ribosomal protein S11, 40S ribosomal protein S15, ... (39 entities in total)
• 機能のキーワード: ribosome, rrna modifications, post-translational modifications, cryo-em
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 35
• 化学式量合計: 1231506.53

構造登録者

Pellegrino, S.,Dent, K.C.,Spikes, T.,Warren, A.J. (登録日: 2022-02-09, 公開日: 2023-02-22, 最終更新日: 2024-04-24)

主引用文献

Pellegrino, S.,Dent, K.C.,Spikes, T.,Warren, A.J.
Cryo-EM reconstruction of the human 40S ribosomal subunit at 2.15 angstrom resolution.
Nucleic Acids Res., 51:4043-4054, 2023

PubMed Abstract: The chemical modification of ribosomal RNA and proteins is critical for ribosome assembly, for protein synthesis and may drive ribosome specialisation in development and disease. However, the inability to accurately visualise these modifications has limited mechanistic understanding of the role of these modifications in ribosome function. Here we report the 2.15 Å resolution cryo-EM reconstruction of the human 40S ribosomal subunit. We directly visualise post-transcriptional modifications within the 18S rRNA and four post-translational modifications of ribosomal proteins. Additionally, we interpret the solvation shells in the core regions of the 40S ribosomal subunit and reveal how potassium and magnesium ions establish both universally conserved and eukaryote-specific coordination to promote the stabilisation and folding of key ribosomal elements. This work provides unprecedented structural details for the human 40S ribosomal subunit that will serve as an important reference for unravelling the functional role of ribosomal RNA modifications.

PubMed: 36951107
DOI: 10.1093/nar/gkad194

実験手法

ELECTRON MICROSCOPY (2.15 Å)