7R1Z

C-terminal domain of hArc in complex with nanobodies H11 and C11, collapsed crystal form

7R1Z の概要

• エントリーDOI: 10.2210/pdb7r1z/pdb
• 分子名称: NbArc-H11, Activity-regulated cytoskeleton-associated protein, NbArc-C11, ... (4 entities in total)
• 機能のキーワード: arc, nanobody, signaling, plasticity, signaling protein
• 由来する生物種: Vicugna pacos; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 48949.57

構造登録者

Markusson, S.,Kursula, P. (登録日: 2022-02-03, 公開日: 2022-06-01, 最終更新日: 2024-10-23)

主引用文献

Markusson, S.,Hallin, E.I.,Bustad, H.J.,Raasakka, A.,Xu, J.,Muruganandam, G.,Loris, R.,Martinez, A.,Bramham, C.R.,Kursula, P.
High-affinity anti-Arc nanobodies provide tools for structural and functional studies.
Plos One, 17:e0269281-e0269281, 2022

PubMed Abstract: Activity-regulated cytoskeleton-associated protein (Arc) is a multidomain protein of retroviral origin with a vital role in the regulation of synaptic plasticity and memory formation in mammals. However, the mechanistic and structural basis of Arc function is poorly understood. Arc has an N-terminal domain (NTD) involved in membrane binding and a C-terminal domain (CTD) that binds postsynaptic protein ligands. In addition, the NTD and CTD both function in Arc oligomerisation, including assembly of retrovirus-like capsids involved in intercellular signalling. To obtain new tools for studies on Arc structure and function, we produced and characterised six high-affinity anti-Arc nanobodies (Nb). The CTD of rat and human Arc were both crystallised in ternary complexes with two Nbs. One Nb bound deep into the stargazin-binding pocket of Arc CTD and suggested competitive binding with Arc ligand peptides. The crystallisation of the human Arc CTD in two different conformations, accompanied by SAXS data and molecular dynamics simulations, paints a dynamic picture of the mammalian Arc CTD. The collapsed conformation closely resembles Drosophila Arc in capsids, suggesting that we have trapped a capsid-like conformation of the human Arc CTD. Our data obtained with the help of anti-Arc Nbs suggest that structural dynamics of the CTD and dimerisation of the NTD may promote the formation of capsids. Taken together, the recombinant high-affinity anti-Arc Nbs are versatile tools that can be further developed for studying mammalian Arc structure and function, as well as mechanisms of Arc capsid formation, both in vitro and in vivo. For example, the Nbs could serve as a genetically encoded tools for inhibition of endogenous Arc interactions in the study of neuronal function and plasticity.

PubMed: 35671319
DOI: 10.1371/journal.pone.0269281

実験手法

X-RAY DIFFRACTION (1.94 Å)