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7R1D

Structure of MuvB complex

Summary for 7R1D
Entry DOI10.2210/pdb7r1d/pdb
EMDB information14239
DescriptorProtein lin-9 homolog, Histone-binding protein RBBP4, Protein lin-37 homolog (3 entities in total)
Functional Keywordsquiescence, transcription, cell cycle
Biological sourceHomo sapiens (human)
More
Total number of polymer chains3
Total formula weight139120.43
Authors
Koliopoulos, M.G.,Alfieri, C. (deposition date: 2022-02-02, release date: 2022-08-10, Last modification date: 2025-07-02)
Primary citationKoliopoulos, M.G.,Muhammad, R.,Roumeliotis, T.I.,Beuron, F.,Choudhary, J.S.,Alfieri, C.
Structure of a nucleosome-bound MuvB transcription factor complex reveals DNA remodelling.
Nat Commun, 13:5075-5075, 2022
Cited by
PubMed Abstract: Genes encoding the core cell cycle machinery are transcriptionally regulated by the MuvB family of protein complexes in a cell cycle-specific manner. Complexes of MuvB with the transcription factors B-MYB and FOXM1 activate mitotic genes during cell proliferation. The mechanisms of transcriptional regulation by these complexes are still poorly characterised. Here, we combine biochemical analysis and in vitro reconstitution, with structural analysis by cryo-electron microscopy and cross-linking mass spectrometry, to functionally examine these complexes. We find that the MuvB:B-MYB complex binds and remodels nucleosomes, thereby exposing nucleosomal DNA. This remodelling activity is supported by B-MYB which directly binds the remodelled DNA. Given the remodelling activity on the nucleosome, we propose that the MuvB:B-MYB complex functions as a pioneer transcription factor complex. In this work, we rationalise prior biochemical and cellular studies and provide a molecular framework of interactions on a protein complex that is key for cell cycle regulation.
PubMed: 36038598
DOI: 10.1038/s41467-022-32798-9
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (3.5 Å)
Structure validation

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数据于2025-07-02公开中

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