+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-14239 | |||||||||
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Title | Structure of MuvB complex | |||||||||
Map data | ||||||||||
Sample |
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Function / homology | Function and homology information : / Myb complex / CAF-1 complex / NURF complex / NuRD complex / regulation of cell fate specification / negative regulation of stem cell population maintenance / DNA replication-dependent chromatin assembly / ESC/E(Z) complex / Transcription of E2F targets under negative control by p107 (RBL1) and p130 (RBL2) in complex with HDAC1 ...: / Myb complex / CAF-1 complex / NURF complex / NuRD complex / regulation of cell fate specification / negative regulation of stem cell population maintenance / DNA replication-dependent chromatin assembly / ESC/E(Z) complex / Transcription of E2F targets under negative control by p107 (RBL1) and p130 (RBL2) in complex with HDAC1 / regulation of stem cell differentiation / Transcription of E2F targets under negative control by DREAM complex / Polo-like kinase mediated events / ATPase complex / positive regulation of stem cell population maintenance / G1/S-Specific Transcription / DNA biosynthetic process / Sin3-type complex / Transcriptional Regulation by E2F6 / RNA Polymerase I Transcription Initiation / histone deacetylase complex / G0 and Early G1 / Cyclin E associated events during G1/S transition / Cyclin A:Cdk2-associated events at S phase entry / Deposition of new CENPA-containing nucleosomes at the centromere / Regulation of TP53 Activity through Acetylation / transcription repressor complex / negative regulation of cell migration / ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression / PRC2 methylates histones and DNA / Regulation of PTEN gene transcription / Defective pyroptosis / HDACs deacetylate histones / negative regulation of transforming growth factor beta receptor signaling pathway / brain development / PKMTs methylate histone lysines / nucleosome assembly / Activation of anterior HOX genes in hindbrain development during early embryogenesis / HCMV Early Events / histone deacetylase binding / histone binding / Oxidative Stress Induced Senescence / Potential therapeutics for SARS / DNA replication / chromosome, telomeric region / regulation of cell cycle / chromatin remodeling / cell cycle / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of cell population proliferation / negative regulation of DNA-templated transcription / DNA-templated transcription / chromatin / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / nucleoplasm / nucleus / cytosol Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.5 Å | |||||||||
Authors | Koliopoulos MG / Alfieri C | |||||||||
Funding support | United Kingdom, 1 items
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Citation | Journal: Nat Commun / Year: 2022 Title: Structure of a nucleosome-bound MuvB transcription factor complex reveals DNA remodelling. Authors: Marios G Koliopoulos / Reyhan Muhammad / Theodoros I Roumeliotis / Fabienne Beuron / Jyoti S Choudhary / Claudio Alfieri / Abstract: Genes encoding the core cell cycle machinery are transcriptionally regulated by the MuvB family of protein complexes in a cell cycle-specific manner. Complexes of MuvB with the transcription factors ...Genes encoding the core cell cycle machinery are transcriptionally regulated by the MuvB family of protein complexes in a cell cycle-specific manner. Complexes of MuvB with the transcription factors B-MYB and FOXM1 activate mitotic genes during cell proliferation. The mechanisms of transcriptional regulation by these complexes are still poorly characterised. Here, we combine biochemical analysis and in vitro reconstitution, with structural analysis by cryo-electron microscopy and cross-linking mass spectrometry, to functionally examine these complexes. We find that the MuvB:B-MYB complex binds and remodels nucleosomes, thereby exposing nucleosomal DNA. This remodelling activity is supported by B-MYB which directly binds the remodelled DNA. Given the remodelling activity on the nucleosome, we propose that the MuvB:B-MYB complex functions as a pioneer transcription factor complex. In this work, we rationalise prior biochemical and cellular studies and provide a molecular framework of interactions on a protein complex that is key for cell cycle regulation. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_14239.map.gz | 2.2 MB | EMDB map data format | |
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Header (meta data) | emd-14239-v30.xml emd-14239.xml | 12.2 KB 12.2 KB | Display Display | EMDB header |
Images | emd_14239.png | 99 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-14239 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-14239 | HTTPS FTP |
-Related structure data
Related structure data | 7r1dMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_14239.map.gz / Format: CCP4 / Size: 38.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.134 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : MuvB complex
Entire | Name: MuvB complex |
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Components |
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-Supramolecule #1: MuvB complex
Supramolecule | Name: MuvB complex / type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Trichoplusia ni (cabbage looper) |
Molecular weight | Experimental: 180 KDa |
-Macromolecule #1: Protein lin-9 homolog
Macromolecule | Name: Protein lin-9 homolog / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 62.03566 KDa |
Recombinant expression | Organism: Trichoplusia ni (cabbage looper) |
Sequence | String: MAELDQLPDE SSSAKALVSL KEGSLSNTWN EKYSSLQKTP VWKGRNTSSA VEMPFRNSKR SRLFSDEDDR QINTRSPKRN QRVAMVPQK FTATMSTPDK KASQKIGFRL RNLLKLPKAH KWCIYEWFYS NIDKPLFEGD NDFCVCLKES FPNLKTRKLT R VEWGKIRR ...String: MAELDQLPDE SSSAKALVSL KEGSLSNTWN EKYSSLQKTP VWKGRNTSSA VEMPFRNSKR SRLFSDEDDR QINTRSPKRN QRVAMVPQK FTATMSTPDK KASQKIGFRL RNLLKLPKAH KWCIYEWFYS NIDKPLFEGD NDFCVCLKES FPNLKTRKLT R VEWGKIRR LMGKPRRCSS AFFEEERSAL KQKRQKIRLL QQRKVADVSQ FKDLPDEIPL PLVIGTKVTA RLRGVHDGLF TG QIDAVDT LNATYRVTFD RTGLGTHTIP DYEVLSNEPH ETMPIAAFGQ KQRPSRFFMT PPRLHYTPPL QSPIIDNDPL LGQ SPWRSK ISGSDTETLG GFPVEFLIQV TRLSKILMIK KEHIKKLREM NTEAEKLKSY SMPISIEFQR RYATIVLELE QLNK DLNKV LHKVQQYCYE LAPDQGLQPA DQPTDMRRRC EEEAQEIVRH ANSSTGQPCV ENENLTDLIS RLTAILLQIK CLAEG GDLN SFEFKSLTDS LNDIKSTIDA SNISCFQNNV EIHVAHIQSG LSQMGNLHAF AANNTNRD |
-Macromolecule #2: Histone-binding protein RBBP4
Macromolecule | Name: Histone-binding protein RBBP4 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 47.709527 KDa |
Recombinant expression | Organism: Trichoplusia ni (cabbage looper) |
Sequence | String: MADKEAAFDD AVEERVINEE YKIWKKNTPF LYDLVMTHAL EWPSLTAQWL PDVTRPEGKD FSIHRLVLGT HTSDEQNHLV IASVQLPND DAQFDASHYD SEKGEFGGFG SVSGKIEIEI KINHEGEVNR ARYMPQNPCI IATKTPSSDV LVFDYTKHPS K PDPSGECN ...String: MADKEAAFDD AVEERVINEE YKIWKKNTPF LYDLVMTHAL EWPSLTAQWL PDVTRPEGKD FSIHRLVLGT HTSDEQNHLV IASVQLPND DAQFDASHYD SEKGEFGGFG SVSGKIEIEI KINHEGEVNR ARYMPQNPCI IATKTPSSDV LVFDYTKHPS K PDPSGECN PDLRLRGHQK EGYGLSWNPN LSGHLLSASD DHTICLWDIS AVPKEGKVVD AKTIFTGHTA VVEDVSWHLL HE SLFGSVA DDQKLMIWDT RSNNTSKPSH SVDAHTAEVN CLSFNPYSEF ILATGSADKT VALWDLRNLK LKLHSFESHK DEI FQVQWS PHNETILASS GTDRRLNVWD LSKIGEEQSP EDAEDGPPEL LFIHGGHTAK ISDFSWNPNE PWVICSVSED NIMQ VWQMA ENIYNDEDPE GSVDPEGQGS |
-Macromolecule #3: Protein lin-37 homolog
Macromolecule | Name: Protein lin-37 homolog / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 29.37524 KDa |
Recombinant expression | Organism: Trichoplusia ni (cabbage looper) |
Sequence | String: MFPVKVKVEK SELEMAKARN QLDAVLQCLL EKSHMDRERL DEEAGKTPSD THNKDCSIAA TGKRPSARFP HQRRKKRREM DDGLAEGGP QRSNTYVIKL FDRSVDLAQF SENTPLYPIC RAWMRNSPSV RERECSPSSP LPPLPEDEEG SEVTNSKSRD V YKLPPPTP ...String: MFPVKVKVEK SELEMAKARN QLDAVLQCLL EKSHMDRERL DEEAGKTPSD THNKDCSIAA TGKRPSARFP HQRRKKRREM DDGLAEGGP QRSNTYVIKL FDRSVDLAQF SENTPLYPIC RAWMRNSPSV RERECSPSSP LPPLPEDEEG SEVTNSKSRD V YKLPPPTP PGPPGDACRS RIPSPLQPEM QGTPDDEPSE PEPSPSTLIY RNMQRWKRIR QRWKEASHRN QLRYSESMKI LR EMYERQG SALEVLFQ |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Grid | Material: COPPER |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | TFS GLACIOS |
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Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 1.6 µm / Nominal defocus min: 0.6 µm |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 60.0 e/Å2 |
-Image processing
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
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Final angle assignment | Type: ANGULAR RECONSTITUTION |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 26641 |