7R07

Abortive infection DNA polymerase AbiK from Lactococcus lactis

7R07 の概要

• エントリーDOI: 10.2210/pdb7r07/pdb
• 関連するPDBエントリー: 7R06 7R08 7Z0Z
• 分子名称: AbiK, DNA (5'-D(*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*C)-3'), MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: dna polymerase, protein-primed dna synthesis, template-independent dna synthesis, abortive infection, antiviral protein
• 由来する生物種: Lactococcus lactis; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 449313.51

構造登録者

Figiel, M.,Gapinska, M.,Czarnocki-Cieciura, M.,Zajko, W.,Nowotny, M. (登録日: 2022-02-01, 公開日: 2022-09-07, 最終更新日: 2024-10-16)

主引用文献

Figiel, M.,Gapinska, M.,Czarnocki-Cieciura, M.,Zajko, W.,Sroka, M.,Skowronek, K.,Nowotny, M.
Mechanism of protein-primed template-independent DNA synthesis by Abi polymerases.
Nucleic Acids Res., 50:10026-10040, 2022

PubMed Abstract: Abortive infection (Abi) is a bacterial antiphage defense strategy involving suicide of the infected cell. Some Abi pathways involve polymerases that are related to reverse transcriptases. They are unique in the way they combine the ability to synthesize DNA in a template-independent manner with protein priming. Here, we report crystal and cryo-electron microscopy structures of two Abi polymerases: AbiK and Abi-P2. Both proteins adopt a bilobal structure with an RT-like domain that comprises palm and fingers subdomains and a unique helical domain. AbiK and Abi-P2 adopt a hexameric and trimeric configuration, respectively, which is unprecedented for reverse transcriptases. Biochemical experiments showed that the formation of these oligomers is required for the DNA polymerization activity. The structure of the AbiK-DNA covalent adduct visualized interactions between the 3' end of DNA and the active site and covalent attachment of the 5' end of DNA to a tyrosine residue used for protein priming. Our data reveal a structural basis of the mechanism of highly unusual template-independent protein-priming polymerases.

PubMed: 36107766
DOI: 10.1093/nar/gkac772

実験手法

X-RAY DIFFRACTION (3.1 Å)