7QZP

Identification and characterization of an RRM-containing, ELAV-like, RNA binding protein in Acinetobacter Baumannii

Summary for 7QZP

• Entry DOI: 10.2210/pdb7qzp/pdb
• Related: 1fxl
• Descriptor: Hypothetical RNA binding protein from Acinetobacter baumannii (2 entities in total)
• Functional Keywords: rna, acinetobacter, rrm, hur, rna binding protein
• Biological source: Acinetobacter baumannii
• Total number of polymer chains: 1
• Total formula weight: 10975.10

Authors

Ciani, C.,Perez-Rafols, A.,Bonomo, I.,Micaelli, M.,Esposito, A.,Zucal, C.,Belli, R.,D'Agostino, V.G.,Bianconi, I.,Calderone, V.,Cerofolini, L.,Fragai, M.,Provenzani, A. (deposition date: 2022-01-31, release date: 2022-07-13, Last modification date: 2024-01-31)

Primary citation

Ciani, C.,Perez-Rafols, A.,Bonomo, I.,Micaelli, M.,Esposito, A.,Zucal, C.,Belli, R.,D'Agostino, V.G.,Bianconi, I.,Calderone, V.,Cerofolini, L.,Massidda, O.,Whalen, M.B.,Fragai, M.,Provenzani, A.
Identification and Characterization of an RRM-Containing, RNA Binding Protein in Acinetobacter baumannii .
Biomolecules, 12:-, 2022

PubMed Abstract: is a Gram-negative pathogen, known to acquire resistance to antibiotics used in the clinic. The RNA-binding proteome of this bacterium is poorly characterized, in particular for what concerns the proteins containing RNA Recognition Motif (RRM). Here, we browsed the proteome for homologous proteins to the human HuR(ELAVL1), an RNA binding protein containing three RRMs. We identified a unique locus that we called -, coding for a protein with a single RRM with an average of 34% identity to the first HuR RRM. We also widen the research to the genomes of all the bacteria, finding 227 entries in 12 bacterial phyla. Notably we observed a partial evolutionary divergence between the RNP1 and RNP2 conserved regions present in the prokaryotes in comparison to the metazoan consensus sequence. We checked the expression at the transcript and protein level, cloned the gene and expressed the recombinant protein. The X-ray and NMR structural characterization of the recombinant AB-Elavl revealed that the protein maintained the typical βαββαβ and three-dimensional organization of eukaryotic RRMs. The biochemical analyses showed that, although the RNP1 and RNP2 show differences, it can bind to AU-rich regions like the human HuR, but with less specificity and lower affinity. Therefore, we identified an RRM-containing RNA-binding protein actually expressed in .

PubMed: 35883478
DOI: 10.3390/biom12070922

Experimental method

X-RAY DIFFRACTION (1.65 Å)