7QZD

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikF with an engineered HMA domain of Pikp-1 (Pikp-SNK-EKE) from rice (Oryza sativa)

7QZD の概要

• エントリーDOI: 10.2210/pdb7qzd/pdb
• 分子名称: Resistance protein Pikp-1, Avr-Pik (3 entities in total)
• 機能のキーワード: plant immunity, pathogen effector, protein engineering, blast disease, antifungal protein
• 由来する生物種: Oryza sativa Japonica Group (Japanese rice); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 55389.98

構造登録者

Maidment, J.H.R.,Franceschetti, M.,Longya, A.,Banfield, M.J. (登録日: 2022-01-31, 公開日: 2022-06-22, 最終更新日: 2024-02-07)

主引用文献

Maidment, J.H.R.,Shimizu, M.,Bentham, A.R.,Vera, S.,Franceschetti, M.,Longya, A.,Stevenson, C.E.M.,De la Concepcion, J.C.,Bialas, A.,Kamoun, S.,Terauchi, R.,Banfield, M.J.
Effector target-guided engineering of an integrated domain expands the disease resistance profile of a rice NLR immune receptor.
Elife, 12:-, 2023

PubMed Abstract: A subset of plant intracellular NLR immune receptors detect effector proteins, secreted by phytopathogens to promote infection, through unconventional integrated domains which resemble the effector's host targets. Direct binding of effectors to these integrated domains activates plant defenses. The rice NLR receptor Pik-1 binds the effector AVR-Pik through an integrated heavy metal-associated (HMA) domain. However, the stealthy alleles AVR-PikC and AVR-PikF avoid interaction with Pik-HMA and evade host defenses. Here, we exploited knowledge of the biochemical interactions between AVR-Pik and its host target, OsHIPP19, to engineer novel Pik-1 variants that respond to AVR-PikC/F. First, we exchanged the HMA domain of Pikp-1 for OsHIPP19-HMA, demonstrating that effector targets can be incorporated into NLR receptors to provide novel recognition profiles. Second, we used the structure of OsHIPP19-HMA to guide the mutagenesis of Pikp-HMA to expand its recognition profile. We demonstrate that the extended recognition profiles of engineered Pikp-1 variants correlate with effector binding in planta and in vitro, and with the gain of new contacts across the effector/HMA interface. Crucially, transgenic rice producing the engineered Pikp-1 variants was resistant to blast fungus isolates carrying AVR-PikC or AVR-PikF. These results demonstrate that effector target-guided engineering of NLR receptors can provide new-to-nature disease resistance in crops.

PubMed: 37199729
DOI: 10.7554/eLife.81123

実験手法

X-RAY DIFFRACTION (2.2 Å)