7QXR

Azacoelenterazine-bound Renilla-type luciferase (AncFT)

Summary for 7QXR

Entry DOI	10.2210/pdb7qxr/pdb
Descriptor	Fragment transplantation onto a hyperstable ancestor of haloalkane dehalogenases and Renilla luciferase (Anc-FT), 3-(4-hydroxyphenyl)-8-[(4-hydroxyphenyl)methyl]-5-(phenylmethyl)-1$l^{4},4,7,8-tetrazabicyclo[4.3.0]nona-1(6),2,4-trien-9-one (3 entities in total)
Functional Keywords	luciferase, azacoelenterazine, substrate analogue, renilla-like, engineered ancestor, oxidoreductase
Biological source	synthetic construct
Total number of polymer chains	3
Total formula weight	103211.54
Authors	Marek, M.,Schenkmayerova, A.,Janin, Y.L. (deposition date: 2022-01-27, release date: 2022-12-07, Last modification date: 2024-01-31)
Primary citation	Schenkmayerova, A.,Toul, M.,Pluskal, D.,Baatallah, R.,Gagnot, G.,Pinto, G.P.,Santana, V.T.,Stuchla, M.,Neugebauer, P.,Chaiyen, P.,Damborsky, J.,Bednar, D.,Janin, Y.L.,Prokop, Z.,Marek, M. Catalytic mechanism for Renilla-type luciferases Nat Catal, 2023 Cited by DOI: 10.1038/s41929-022-00895-z PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.052 Å)

Structure validation