7QXM

Crystal structure of the Vibrio cholerae replicative helicase (DnaB)

7QXM の概要

• エントリーDOI: 10.2210/pdb7qxm/pdb
• 関連するPDBエントリー: 6T66
• 分子名称: Replicative DNA helicase (1 entity in total)
• 機能のキーワード: helicase, replication
• 由来する生物種: Vibrio cholerae
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 317625.87

構造登録者

Legrand, P.,Quevillon-Cheruel, S.,Walbott, H.,Cargemel, C. (登録日: 2022-01-26, 公開日: 2022-05-25, 最終更新日: 2024-01-31)

主引用文献

Cargemel, C.,Walbott, H.,Durand, D.,Legrand, P.,Ouldali, M.,Ferat, J.L.,Marsin, S.,Quevillon-Cheruel, S.
The apo-form of the Vibrio cholerae replicative helicase DnaB is a labile and inactive planar trimer of dimers.
Febs Lett., 596:2031-2040, 2022

PubMed Abstract: To enable chromosomal replication, DNA is unwound by the ATPase molecular motor replicative helicase. The bacterial helicase DnaB is a ring-shaped homo-hexamer whose conformational dynamics are being studied through its different 3D structural states adopted along its functional cycle. Our findings describe a new crystal structure for the apo-DnaB from Vibrio cholerae, forming a planar hexamer with pseudo-symmetry, constituted by a trimer of dimers in which the C-terminal domains delimit a triskelion-shaped hole. This hexamer is labile and inactive. We suggest that it represents an intermediate state allowing the formation of the active NTP-bound hexamer from dimers.

PubMed: 35568982
DOI: 10.1002/1873-3468.14403

実験手法

X-RAY DIFFRACTION (3.8 Å)