7QVE

Spinach 20S proteasome

7QVE の概要

• エントリーDOI: 10.2210/pdb7qve/pdb
• EMDBエントリー: 14175
• 分子名称: Proteasome subunit alpha type, Proteasome subunit beta, Proteasome subunit beta type-5, ... (14 entities in total)
• 機能のキーワード: proteasome, ups, plant, spinach, plant protein
• 由来する生物種: Spinacia oleracea (spinach); 詳細
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 745085.78

構造登録者

Kandolf, S.,Grishkovskaya, I.,Meinhart, A.,Haselbach, D. (登録日: 2022-01-21, 公開日: 2022-05-25, 最終更新日: 2024-07-17)

主引用文献

Kandolf, S.,Grishkovskaya, I.,Belacic, K.,Bolhuis, D.L.,Amann, S.,Foster, B.,Imre, R.,Mechtler, K.,Schleiffer, A.,Tagare, H.D.,Zhong, E.D.,Meinhart, A.,Brown, N.G.,Haselbach, D.
Cryo-EM structure of the plant 26S proteasome.
Plant Commun., 3:100310-100310, 2022

PubMed Abstract: Targeted proteolysis is a hallmark of life. It is especially important in long-lived cells that can be found in higher eukaryotes, like plants. This task is mainly fulfilled by the ubiquitin-proteasome system. Thus, proteolysis by the 26S proteasome is vital to development, immunity, and cell division. Although the yeast and animal proteasomes are well characterized, there is only limited information on the plant proteasome. We determined the first plant 26S proteasome structure from Spinacia oleracea by single-particle electron cryogenic microscopy at an overall resolution of 3.3 Å. We found an almost identical overall architecture of the spinach proteasome compared with the known structures from mammals and yeast. Nevertheless, we noticed a structural difference in the proteolytic active β1 subunit. Furthermore, we uncovered an unseen compression state by characterizing the proteasome's conformational landscape. We suspect that this new conformation of the 20S core protease, in correlation with a partial opening of the unoccupied gate, may contribute to peptide release after proteolysis. Our data provide a structural basis for the plant proteasome, which is crucial for further studies.

PubMed: 35576154
DOI: 10.1016/j.xplc.2022.100310

実験手法

ELECTRON MICROSCOPY (3.3 Å)