7QUT

serial synchrotron crystallographic structure of Drosophila Melanogaster (6-4) photolyase

Summary for 7QUT

Entry DOI	10.2210/pdb7qut/pdb
Descriptor	RE11660p, FLAVIN-ADENINE DINUCLEOTIDE, GLYCEROL, ... (4 entities in total)
Functional Keywords	flavoprotein, (6-4)photolyase, dna binding protein
Biological source	Drosophila melanogaster (fruit fly)
Total number of polymer chains	1
Total formula weight	59725.33
Authors	Cellini, A.,Weixiao, Y.W.,Kumar, M.S.,Westenhoff, S. (deposition date: 2022-01-18, release date: 2022-04-13, Last modification date: 2024-01-31)
Primary citation	Cellini, A.,Shankar, M.K.,Wahlgren, W.Y.,Nimmrich, A.,Furrer, A.,James, D.,Wranik, M.,Aumonier, S.,Beale, E.V.,Dworkowski, F.,Standfuss, J.,Weinert, T.,Westenhoff, S. Structural basis of the radical pair state in photolyases and cryptochromes. Chem.Commun.(Camb.), 58:4889-4892, 2022 Cited by PubMed Abstract: We present the structure of a photoactivated animal (6-4) photolyase in its radical pair state, captured by serial crystallography. We observe how a conserved asparigine moves towards the semiquinone FAD chromophore and stabilizes it by hydrogen bonding. Several amino acids around the final tryptophan radical rearrange, opening it up to the solvent. The structure explains how the protein environment stabilizes the radical pair state, which is crucial for function of (6-4) photolyases and cryptochromes. PubMed: 35352724 DOI: 10.1039/d2cc00376g PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.24 Å)

Structure validation