7QUT

serial synchrotron crystallographic structure of Drosophila Melanogaster (6-4) photolyase

7QUT の概要

• エントリーDOI: 10.2210/pdb7qut/pdb
• 分子名称: RE11660p, FLAVIN-ADENINE DINUCLEOTIDE, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: flavoprotein, (6-4)photolyase, dna binding protein
• 由来する生物種: Drosophila melanogaster (fruit fly)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 59725.33

構造登録者

Cellini, A.,Weixiao, Y.W.,Kumar, M.S.,Westenhoff, S. (登録日: 2022-01-18, 公開日: 2022-04-13, 最終更新日: 2024-01-31)

主引用文献

Cellini, A.,Shankar, M.K.,Wahlgren, W.Y.,Nimmrich, A.,Furrer, A.,James, D.,Wranik, M.,Aumonier, S.,Beale, E.V.,Dworkowski, F.,Standfuss, J.,Weinert, T.,Westenhoff, S.
Structural basis of the radical pair state in photolyases and cryptochromes.
Chem.Commun.(Camb.), 58:4889-4892, 2022

PubMed Abstract: We present the structure of a photoactivated animal (6-4) photolyase in its radical pair state, captured by serial crystallography. We observe how a conserved asparigine moves towards the semiquinone FAD chromophore and stabilizes it by hydrogen bonding. Several amino acids around the final tryptophan radical rearrange, opening it up to the solvent. The structure explains how the protein environment stabilizes the radical pair state, which is crucial for function of (6-4) photolyases and cryptochromes.

PubMed: 35352724
DOI: 10.1039/d2cc00376g

実験手法

X-RAY DIFFRACTION (2.24 Å)