7QR8
SpCas9 bound to PTPRC off-target1 DNA substrate
Summary for 7QR8
Entry DOI | 10.2210/pdb7qr8/pdb |
Descriptor | PTPRC sgRNA, CRISPR-associated endonuclease Cas9/Csn1, PTPRC off-target1 target strand, ... (7 entities in total) |
Functional Keywords | crispr, cas9, off-target, ternary complex, hydrolase |
Biological source | Streptococcus pyogenes More |
Total number of polymer chains | 4 |
Total formula weight | 198728.69 |
Authors | Pacesa, M.,Jinek, M. (deposition date: 2022-01-10, release date: 2022-10-26, Last modification date: 2024-01-31) |
Primary citation | Pacesa, M.,Lin, C.H.,Clery, A.,Saha, A.,Arantes, P.R.,Bargsten, K.,Irby, M.J.,Allain, F.H.,Palermo, G.,Cameron, P.,Donohoue, P.D.,Jinek, M. Structural basis for Cas9 off-target activity. Cell, 185:4067-4081.e21, 2022 Cited by PubMed: 36306733DOI: 10.1016/j.cell.2022.09.026 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.75 Å) |
Structure validation
Download full validation report