7QPU

Botulinum neurotoxin A5 cell binding domain in complex with GM1b oligosaccharide

7QPU の概要

• エントリーDOI: 10.2210/pdb7qpu/pdb
• 分子名称: Botulinum neurotoxin sub-type A5, N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose, DI(HYDROXYETHYL)ETHER, ... (5 entities in total)
• 機能のキーワード: cell binding domain, receptor, oligosaccharide, neurotoxin, toxin
• 由来する生物種: Clostridium botulinum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 102112.43

構造登録者

Gregory, K.S.,Acharya, K.R.,Liu, S.M. (登録日: 2022-01-05, 公開日: 2022-03-09, 最終更新日: 2024-01-31)

主引用文献

Gregory, K.S.,Mojanaga, O.O.,Liu, S.M.,Acharya, K.R.
Crystal Structures of Botulinum Neurotoxin Subtypes A4 and A5 Cell Binding Domains in Complex with Receptor Ganglioside.
Toxins, 14:-, 2022

PubMed Abstract: Botulinum neurotoxins (BoNT) cause the potentially fatal neuroparalytic disease botulism that arises due to proteolysis of a SNARE protein. Each BoNT is comprised of three domains: a cell binding domain (H), a translocation domain (H), and a catalytic (Zn endopeptidase) domain (LC). The H is responsible for neuronal specificity by targeting both a protein and ganglioside receptor at the neuromuscular junction. Although highly toxic, some BoNTs are commercially available as therapeutics for the treatment of a range of neuromuscular conditions. Here we present the crystal structures of two BoNT cell binding domains, H/A4 and H/A5, in a complex with the oligosaccharide of ganglioside, GD1a and GM1b, respectively. These structures, along with a detailed comparison with the previously reported apo-structures, reveal the conformational changes that occur upon ganglioside binding and the interactions involved.

PubMed: 35202156
DOI: 10.3390/toxins14020129

実験手法

X-RAY DIFFRACTION (2.4 Å)