7QPG

Human RZZ kinetochore corona complex.

7QPG の概要

• エントリーDOI: 10.2210/pdb7qpg/pdb
• EMDBエントリー: 14120
• 分子名称: Protein zwilch homolog, Kinetochore-associated protein 1, Centromere/kinetochore protein zw10 homolog (3 entities in total)
• 機能のキーワード: kinetochore, centromere, chromosome segregation, mitosis, cell cycle
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 872079.41

構造登録者

Raisch, T.,Ciossani, G.,d'Amico, E.,Cmetowski, V.,Carmignani, S.,Maffini, S.,Merino, F.,Wohlgemuth, S.,Vetter, I.R.,Raunser, S.,Musacchio, A. (登録日: 2022-01-04, 公開日: 2022-03-16, 最終更新日: 2024-07-17)

主引用文献

Raisch, T.,Ciossani, G.,d'Amico, E.,Cmentowski, V.,Carmignani, S.,Maffini, S.,Merino, F.,Wohlgemuth, S.,Vetter, I.R.,Raunser, S.,Musacchio, A.
Structure of the RZZ complex and molecular basis of Spindly-driven corona assembly at human kinetochores.
Embo J., 41:e110411-e110411, 2022

PubMed Abstract: In metazoans, a ≈1 megadalton (MDa) multiprotein complex comprising the dynein-dynactin adaptor Spindly and the ROD-Zwilch-ZW10 (RZZ) complex is the building block of a fibrous biopolymer, the kinetochore fibrous corona. The corona assembles on mitotic kinetochores to promote microtubule capture and spindle assembly checkpoint (SAC) signaling. We report here a high-resolution cryo-EM structure that captures the essential features of the RZZ complex, including a farnesyl-binding site required for Spindly binding. Using a highly predictive in vitro assay, we demonstrate that the SAC kinase MPS1 is necessary and sufficient for corona assembly at supercritical concentrations of the RZZ-Spindly (RZZS) complex, and describe the molecular mechanism of phosphorylation-dependent filament nucleation. We identify several structural requirements for RZZS polymerization in rings and sheets. Finally, we identify determinants of kinetochore localization and corona assembly of Spindly. Our results describe a framework for the long-sought-for molecular basis of corona assembly on metazoan kinetochores.

PubMed: 35373361
DOI: 10.15252/embj.2021110411

実験手法

ELECTRON MICROSCOPY (3.9 Å)