7QP7

Structure of the human 48S initiation complex in closed state (h48S AUG closed)

これはPDB形式変換不可エントリーです。

7QP7 の概要

• エントリーDOI: 10.2210/pdb7qp7/pdb
• EMDBエントリー: 14114
• 分子名称: Eukaryotic translation initiation factor 3 subunit B, 40S ribosomal protein S11, 40S ribosomal protein S4, X isoform, ... (52 entities in total)
• 機能のキーワード: 48s, initiation, eif3, ternary complex, translation, open state, ribosome
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 51
• 化学式量合計: 2165279.14

構造登録者

Yi, S.-H.,Petrychenko, V.,Schliep, J.E.,Goyal, A.,Linden, A.,Chari, A.,Urlaub, H.,Stark, H.,Rodnina, M.V.,Adio, S.,Fischer, N. (登録日: 2022-01-03, 公開日: 2022-05-11, 最終更新日: 2024-04-24)

主引用文献

Yi, S.H.,Petrychenko, V.,Schliep, J.E.,Goyal, A.,Linden, A.,Chari, A.,Urlaub, H.,Stark, H.,Rodnina, M.V.,Adio, S.,Fischer, N.
Conformational rearrangements upon start codon recognition in human 48S translation initiation complex.
Nucleic Acids Res., 50:5282-5298, 2022

PubMed Abstract: Selection of the translation start codon is a key step during protein synthesis in human cells. We obtained cryo-EM structures of human 48S initiation complexes and characterized the intermediates of codon recognition by kinetic methods using eIF1A as a reporter. Both approaches capture two distinct ribosome populations formed on an mRNA with a cognate AUG codon in the presence of eIF1, eIF1A, eIF2-GTP-Met-tRNAiMet and eIF3. The 'open' 40S subunit conformation differs from the human 48S scanning complex and represents an intermediate preceding the codon recognition step. The 'closed' form is similar to reported structures of complexes from yeast and mammals formed upon codon recognition, except for the orientation of eIF1A, which is unique in our structure. Kinetic experiments show how various initiation factors mediate the population distribution of open and closed conformations until 60S subunit docking. Our results provide insights into the timing and structure of human translation initiation intermediates and suggest the differences in the mechanisms of start codon selection between mammals and yeast.

PubMed: 35489072
DOI: 10.1093/nar/gkac283

実験手法

ELECTRON MICROSCOPY (3.7 Å)