7QIX

Specific features and methylation sites of a plant ribosome. 40S body ribosomal subunit.

7QIX の概要

• エントリーDOI: 10.2210/pdb7qix/pdb
• 関連するPDBエントリー: 7QIW
• EMDBエントリー: 14001 14002
• 分子名称: 18S rRNA body, 40S ribosomal protein S21, 40S body ribosomal protein uS12, ... (26 entities in total)
• 機能のキーワード: solanum lycopersicum, cytosolic ribosome, 80s, plant, rrna, ribosome
• 由来する生物種: Solanum lycopersicum; 詳細
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 988259.54

構造登録者

Cottilli, P.,Itoh, Y.,Amunts, A. (登録日: 2021-12-16, 公開日: 2022-06-15, 最終更新日: 2024-10-23)

主引用文献

Cottilli, P.,Itoh, Y.,Nobe, Y.,Petrov, A.S.,Lison, P.,Taoka, M.,Amunts, A.
Cryo-EM structure and rRNA modification sites of a plant ribosome.
Plant Commun., 3:100342-100342, 2022

PubMed Abstract: Protein synthesis in crop plants contributes to the balance of food and fuel on our planet, which influences human metabolic activity and lifespan. Protein synthesis can be regulated with respect to changing environmental cues via the deposition of chemical modifications into rRNA. Here, we present the structure of a plant ribosome from tomato and a quantitative mass spectrometry analysis of its rRNAs. The study reveals fine features of the ribosomal proteins and 71 plant-specific rRNA modifications, and it re-annotates 30 rRNA residues in the available sequence. At the protein level, isoAsp is found in position 137 of uS11, and a zinc finger previously believed to be universal is missing from eL34, suggesting a lower effect of zinc deficiency on protein synthesis in plants. At the rRNA level, the plant ribosome differs markedly from its human counterpart with respect to the spatial distribution of modifications. Thus, it represents an additional layer of gene expression regulation, highlighting the molecular signature of a plant ribosome. The results provide a reference model of a plant ribosome for structural studies and an accurate marker for molecular ecology.

PubMed: 35643637
DOI: 10.1016/j.xplc.2022.100342

実験手法

ELECTRON MICROSCOPY (2.53 Å)