7QFL

Crystal structure of S-layer protein SlpA from Lactobacillus acidophilus, domain II (aa 199-308)

7QFL の概要

• エントリーDOI: 10.2210/pdb7qfl/pdb
• 分子名称: S-layer protein, PHOSPHATE ION, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: s-layer, surface layer, bacterial, lactobacillus acidophilus, slpa, self-assembly, structural protein
• 由来する生物種: Lactobacillus acidophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14312.64

構造登録者

Sagmeister, T.,Dordic, A.,Eder, E.,Pavkov-Keller, T. (登録日: 2021-12-06, 公開日: 2022-12-21, 最終更新日: 2024-06-19)

主引用文献

Sagmeister, T.,Gubensak, N.,Buhlheller, C.,Grininger, C.,Eder, M.,Ðordic, A.,Millan, C.,Medina, A.,Murcia, P.A.S.,Berni, F.,Hynonen, U.,Vejzovic, D.,Damisch, E.,Kulminskaya, N.,Petrowitsch, L.,Oberer, M.,Palva, A.,Malanovic, N.,Codee, J.,Keller, W.,Uson, I.,Pavkov-Keller, T.
The molecular architecture of Lactobacillus S-layer: Assembly and attachment to teichoic acids.
Proc.Natl.Acad.Sci.USA, 121:e2401686121-e2401686121, 2024

PubMed Abstract: S-layers are crystalline arrays found on bacterial and archaeal cells. is a diverse family of bacteria known especially for potential gut health benefits. This study focuses on the S-layer proteins from and common in the mammalian gut. Atomic resolution structures of S-layer proteins SlpA and SlpX exhibit domain swapping, and the obtained assembly model of the main S-layer protein SlpA aligns well with prior electron microscopy and mutagenesis data. The S-layer's pore size suggests a protective role, with charged areas aiding adhesion. A highly similar domain organization and interaction network are observed across the genus. Interaction studies revealed conserved binding areas specific for attachment to teichoic acids. The structure of the SlpA S-layer and the suggested incorporation of SlpX as well as its interaction with teichoic acids lay the foundation for deciphering its role in immune responses and for developing effective treatments for a variety of infectious and bacteria-mediated inflammation processes, opening opportunities for targeted engineering of the S-layer or lactobacilli bacteria in general.

PubMed: 38838019
DOI: 10.1073/pnas.2401686121

実験手法

X-RAY DIFFRACTION (1.4 Å)