7QD2

Structure of the orange carotenoid protein from Planktothrix agardhii binding canthaxanthin in the P21 space group

7QD2 の概要

• エントリーDOI: 10.2210/pdb7qd2/pdb
• 分子名称: Orange carotenoid-binding protein, beta,beta-carotene-4,4'-dione, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: carotenoid-binding, photoprotection, carotenoid binding protein, photosynthesis
• 由来する生物種: Planktothrix agardhii (Oscillatoria agardhii)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72077.78

構造登録者

Andreeva, E.A.,Hartmann, E.,Schlichting, I.,Colletier, J.-P. (登録日: 2021-11-26, 公開日: 2022-07-06, 最終更新日: 2024-11-13)

主引用文献

Wilson, A.,Andreeva, E.A.,Nizinski, S.J.,Talbot, L.,Hartmann, E.,Schlichting, I.,Burdzinski, G.,Sliwa, M.,Kirilovsky, D.,Colletier, J.P.
Structure-function-dynamics relationships in the peculiar Planktothrix PCC7805 OCP1: Impact of his-tagging and carotenoid type.
Biochim Biophys Acta Bioenerg, 1863:148584-148584, 2022

PubMed Abstract: The orange carotenoid protein (OCP) is a photoactive protein involved in cyanobacterial photoprotection. Here, we report on the functional, spectral and structural characteristics of the peculiar Planktothrix PCC7805 OCP (Plankto-OCP). We show that this OCP variant is characterized by higher photoactivation and recovery rates, and a stronger energy-quenching activity, compared to other OCP studied thus far. We characterize the effect of the functionalizing carotenoid and of his-tagging on these reactions, and identify the time scales on which these modifications affect photoactivation. The presence of a his-tag at the C-terminus has a large influence on photoactivation, thermal recovery and PBS-fluorescence quenching, and likewise for the nature of the carotenoid that additionally affects the yield and characteristics of excited states and the ns-s dynamics of photoactivated OCP. By solving the structures of Plankto-OCP in the ECN- and CAN-functionalized states, each in two closely-related crystal forms, we further unveil the molecular breathing motions that animate Plankto-OCP at the monomer and dimer levels. We finally discuss the structural changes that could explain the peculiar properties of Plankto-OCP.

PubMed: 35752265
DOI: 10.1016/j.bbabio.2022.148584

実験手法

X-RAY DIFFRACTION (1.4 Å)