7QCD

CryoEM structure of the Smc5/6-holocomplex (composite structure)

7QCD の概要

• エントリーDOI: 10.2210/pdb7qcd/pdb
• EMDBエントリー: 13893 13894 13895
• 分子名称: Structural maintenance of chromosomes protein 5, Structural maintenance of chromosomes protein 6, E3 SUMO-protein ligase MMS21, ... (7 entities in total)
• 機能のキーワード: structural maintenance of chromosomes, smc, holo-complex, recombination, stalled replication fork
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 442838.70

構造登録者

Hallett, S.T.,Oliver, A.W. (登録日: 2021-11-23, 公開日: 2022-09-21, 最終更新日: 2024-07-17)

主引用文献

Hallett, S.T.,Campbell Harry, I.,Schellenberger, P.,Zhou, L.,Cronin, N.B.,Baxter, J.,Etheridge, T.J.,Murray, J.M.,Oliver, A.W.
Cryo-EM structure of the Smc5/6 holo-complex.
Nucleic Acids Res., 50:9505-9520, 2022

PubMed Abstract: The Smc5/6 complex plays an essential role in the resolution of recombination intermediates formed during mitosis or meiosis, or as a result of the cellular response to replication stress. It also functions as a restriction factor preventing viral replication. Here, we report the cryogenic EM (cryo-EM) structure of the six-subunit budding yeast Smc5/6 holo-complex, reconstituted from recombinant proteins expressed in insect cells - providing both an architectural overview of the entire complex and an understanding of how the Nse1/3/4 subcomplex binds to the hetero-dimeric SMC protein core. In addition, we demonstrate that a region within the head domain of Smc5, equivalent to the 'W-loop' of Smc4 or 'F-loop' of Smc1, mediates an important interaction with Nse1. Notably, mutations that alter the surface-charge profile of the region of Nse1 which accepts the Smc5-loop, lead to a slow-growth phenotype and a global reduction in the chromatin-associated fraction of the Smc5/6 complex, as judged by single molecule localisation microscopy experiments in live yeast. Moreover, when taken together, our data indicates functional equivalence between the structurally unrelated KITE and HAWK accessory subunits associated with SMC complexes.

PubMed: 35993814
DOI: 10.1093/nar/gkac692

実験手法

ELECTRON MICROSCOPY (8 Å)