7QAR
Serial crystallography structure of cofactor-free urate oxidase in complex with the 5-peroxo derivative of 9-methyl uric acid at room temperature
This is a non-PDB format compatible entry.
Summary for 7QAR
| Entry DOI | 10.2210/pdb7qar/pdb |
| Descriptor | Uricase, (5S)-5-(dioxidanyl)-9-methyl-7H-purine-2,6,8-trione (3 entities in total) |
| Functional Keywords | cofactor-free oxidase, reaction intermediate, serial crystallography, dioxygen, oxidoreductase |
| Biological source | Aspergillus flavus |
| Total number of polymer chains | 1 |
| Total formula weight | 34502.88 |
| Authors | Bui, S.,Catapano, L.,Zielinski, K.,Yefanov, O.,Murshudov, G.N.,Oberthuer, D.,Steiner, R.A. (deposition date: 2021-11-17, release date: 2023-04-26, Last modification date: 2024-02-07) |
| Primary citation | Zielinski, K.A.,Prester, A.,Andaleeb, H.,Bui, S.,Yefanov, O.,Catapano, L.,Henkel, A.,Wiedorn, M.O.,Lorbeer, O.,Crosas, E.,Meyer, J.,Mariani, V.,Domaracky, M.,White, T.A.,Fleckenstein, H.,Sarrou, I.,Werner, N.,Betzel, C.,Rohde, H.,Aepfelbacher, M.,Chapman, H.N.,Perbandt, M.,Steiner, R.A.,Oberthuer, D. Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive. Iucrj, 9:778-791, 2022 Cited by PubMed Abstract: Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus, its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physio-logical temperatures would be a great benefit, is impaired. On the way to high-throughput SSX using a conveyor belt based sample delivery system - the CFEL TapeDrive - with three different proteins of biological relevance ( CTX-M-14 β-lactamase, xylanase GH11 and urate oxidase), it is shown here that complete datasets can be collected in less than a minute and only minimal amounts of sample are required. PubMed: 36381150DOI: 10.1107/S2052252522010193 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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