7QAR

Serial crystallography structure of cofactor-free urate oxidase in complex with the 5-peroxo derivative of 9-methyl uric acid at room temperature

これはPDB形式変換不可エントリーです。

7QAR の概要

• エントリーDOI: 10.2210/pdb7qar/pdb
• 分子名称: Uricase, (5S)-5-(dioxidanyl)-9-methyl-7H-purine-2,6,8-trione (3 entities in total)
• 機能のキーワード: cofactor-free oxidase, reaction intermediate, serial crystallography, dioxygen, oxidoreductase
• 由来する生物種: Aspergillus flavus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34502.88

構造登録者

Bui, S.,Catapano, L.,Zielinski, K.,Yefanov, O.,Murshudov, G.N.,Oberthuer, D.,Steiner, R.A. (登録日: 2021-11-17, 公開日: 2023-04-26, 最終更新日: 2024-02-07)

主引用文献

Zielinski, K.A.,Prester, A.,Andaleeb, H.,Bui, S.,Yefanov, O.,Catapano, L.,Henkel, A.,Wiedorn, M.O.,Lorbeer, O.,Crosas, E.,Meyer, J.,Mariani, V.,Domaracky, M.,White, T.A.,Fleckenstein, H.,Sarrou, I.,Werner, N.,Betzel, C.,Rohde, H.,Aepfelbacher, M.,Chapman, H.N.,Perbandt, M.,Steiner, R.A.,Oberthuer, D.
Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive.
Iucrj, 9:778-791, 2022

PubMed Abstract: Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus, its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physio-logical temperatures would be a great benefit, is impaired. On the way to high-throughput SSX using a conveyor belt based sample delivery system - the CFEL TapeDrive - with three different proteins of biological relevance ( CTX-M-14 β-lactamase, xylanase GH11 and urate oxidase), it is shown here that complete datasets can be collected in less than a minute and only minimal amounts of sample are required.

PubMed: 36381150
DOI: 10.1107/S2052252522010193

実験手法

X-RAY DIFFRACTION (2.3 Å)