7QAN

Cytochrome P450 Enzyme AbyV

これはPDB形式変換不可エントリーです。

7QAN の概要

• エントリーDOI: 10.2210/pdb7qan/pdb
• 分子名称: Cytochrome P450, PROTOPORPHYRIN IX CONTAINING FE, TRIETHYLENE GLYCOL, ... (10 entities in total)
• 機能のキーワード: cytochrome p450, micromonospora maris, abyssomicin, heme, monooxygenase, oxidoreductase
• 由来する生物種: Micromonospora maris (strain DSM 45365 / JCM 31040 / NBRC 109089 / NRRL B-24793 / AB-18-032) (Verrucosispora maris)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 92108.91

構造登録者

Parnell, A.E.,Back, C.R.,Race, P.R. (登録日: 2021-11-17, 公開日: 2022-11-30, 最終更新日: 2024-01-31)

主引用文献

Devine, A.J.,Parnell, A.E.,Back, C.R.,Lees, N.R.,Johns, S.T.,Zulkepli, A.Z.,Barringer, R.,Zorn, K.,Stach, J.E.M.,Crump, M.P.,Hayes, M.A.,van der Kamp, M.W.,Race, P.R.,Willis, C.L.
The Role of Cytochrome P450 AbyV in the Final Stages of Abyssomicin C Biosynthesis.
Angew.Chem.Int.Ed.Engl., 62:e202213053-e202213053, 2023

PubMed Abstract: Abyssomicin C and its atropisomer are potent inhibitors of bacterial folate metabolism. They possess complex polycyclic structures, and their biosynthesis has been shown to involve several unusual enzymatic transformations. Using a combination of synthesis and in vitro assays we reveal that AbyV, a cytochrome P450 enzyme from the aby gene cluster, catalyses a key late-stage epoxidation required for the installation of the characteristic ether-bridged core of abyssomicin C. The X-ray crystal structure of AbyV has been determined, which in combination with molecular dynamics simulations provides a structural framework for our functional data. This work demonstrates the power of combining selective carbon-13 labelling with NMR spectroscopy as a sensitive tool to interrogate enzyme-catalysed reactions in vitro with no need for purification.

PubMed: 36314667
DOI: 10.1002/anie.202213053

実験手法

X-RAY DIFFRACTION (2.01 Å)