7Q8C

Leishmania major actin filament in ADP-state

Summary for 7Q8C

• Entry DOI: 10.2210/pdb7q8c/pdb
• Related: 7Q8B
• EMDB information: 13863 13864
• Descriptor: Actin, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• Functional Keywords: actin, filament, parasite, adp-pi, structural protein
• Biological source: Leishmania major
• Total number of polymer chains: 5
• Total formula weight: 212576.86

Authors

Kotila, T.,Muniyandi, S.,Lappalainen, P.,Huiskonen, J.T. (deposition date: 2021-11-11, release date: 2022-05-18, Last modification date: 2024-07-17)

Primary citation

Kotila, T.,Wioland, H.,Selvaraj, M.,Kogan, K.,Antenucci, L.,Jegou, A.,Huiskonen, J.T.,Romet-Lemonne, G.,Lappalainen, P.
Structural basis of rapid actin dynamics in the evolutionarily divergent Leishmania parasite.
Nat Commun, 13:3442-3442, 2022

PubMed Abstract: Actin polymerization generates forces for cellular processes throughout the eukaryotic kingdom, but our understanding of the 'ancient' actin turnover machineries is limited. We show that, despite > 1 billion years of evolution, pathogenic Leishmania major parasite and mammalian actins share the same overall fold and co-polymerize with each other. Interestingly, Leishmania harbors a simple actin-regulatory machinery that lacks cofilin 'cofactors', which accelerate filament disassembly in higher eukaryotes. By applying single-filament biochemistry we discovered that, compared to mammalian proteins, Leishmania actin filaments depolymerize more rapidly from both ends, and are severed > 100-fold more efficiently by cofilin. Our high-resolution cryo-EM structures of Leishmania ADP-, ADP-Pi- and cofilin-actin filaments identify specific features at actin subunit interfaces and cofilin-actin interactions that explain the unusually rapid dynamics of parasite actin filaments. Our findings reveal how divergent parasites achieve rapid actin dynamics using a remarkably simple set of actin-binding proteins, and elucidate evolution of the actin cytoskeleton.

PubMed: 35705539
DOI: 10.1038/s41467-022-31068-y

Experimental method

ELECTRON MICROSCOPY (2.72 Å)