7Q2X

Cryo-EM structure of clamped S.cerevisiae condensin-DNA complex (Form I)

7Q2X の概要

• エントリーDOI: 10.2210/pdb7q2x/pdb
• EMDBエントリー: 13783
• 分子名称: Structural maintenance of chromosomes protein 2, Structural maintenance of chromosomes protein 4, Condensin complex subunit 2, ... (9 entities in total)
• 機能のキーワード: condensin, smc, cell cycle
• 由来する生物種: Saccharomyces cerevisiae S288C; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 539173.24

構造登録者

Lee, B.-G.,Rhodes, J.,Lowe, J. (登録日: 2021-10-26, 公開日: 2022-03-23, 最終更新日: 2024-11-20)

主引用文献

Lee, B.G.,Rhodes, J.,Lowe, J.
Clamping of DNA shuts the condensin neck gate.
Proc.Natl.Acad.Sci.USA, 119:e2120006119-e2120006119, 2022

PubMed Abstract: SignificanceDNA needs to be compacted to fit into nuclei and during cell division, when dense chromatids are formed for their mechanical segregation, a process that depends on the protein complex condensin. It forms and enlarges loops in DNA through loop extrusion. Our work resolves the atomic structure of a DNA-bound state of condensin in which ATP has not been hydrolyzed. The DNA is clamped within a compartment that has been reported previously in other structural maintenance of chromosomes (SMC) complexes, including Rad50, cohesin, and MukBEF. With the caveat of important differences, it means that all SMC complexes cycle through at least some similar states and undergo similar conformational changes in their head modules, while hydrolyzing ATP and translocating DNA.

PubMed: 35349345
DOI: 10.1073/pnas.2120006119

実験手法

ELECTRON MICROSCOPY (3 Å)