7PZP

Mitochondrial DNA dependent RNA polymerase homodimer.

7PZP の概要

• エントリーDOI: 10.2210/pdb7pzp/pdb
• EMDBエントリー: 13735
• 分子名称: DNA-directed RNA polymerase, mitochondrial (1 entity in total)
• 機能のキーワード: mitochondria, dna dependent rna polymerase, 7s rna, transcription
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 274584.25

構造登録者

Das, H.,Hallberg, B.M. (登録日: 2021-10-13, 公開日: 2022-09-28, 最終更新日: 2024-07-17)

主引用文献

Zhu, X.,Xie, X.,Das, H.,Tan, B.G.,Shi, Y.,Al-Behadili, A.,Peter, B.,Motori, E.,Valenzuela, S.,Posse, V.,Gustafsson, C.M.,Hallberg, B.M.,Falkenberg, M.
Non-coding 7S RNA inhibits transcription via mitochondrial RNA polymerase dimerization.
Cell, 185:2309-, 2022

PubMed Abstract: The mitochondrial genome encodes 13 components of the oxidative phosphorylation system, and altered mitochondrial transcription drives various human pathologies. A polyadenylated, non-coding RNA molecule known as 7S RNA is transcribed from a region immediately downstream of the light strand promoter in mammalian cells, and its levels change rapidly in response to physiological conditions. Here, we report that 7S RNA has a regulatory function, as it controls levels of mitochondrial transcription both in vitro and in cultured human cells. Using cryo-EM, we show that POLRMT dimerization is induced by interactions with 7S RNA. The resulting POLRMT dimer interface sequesters domains necessary for promoter recognition and unwinding, thereby preventing transcription initiation. We propose that the non-coding 7S RNA molecule is a component of a negative feedback loop that regulates mitochondrial transcription in mammalian cells.

PubMed: 35662414
DOI: 10.1016/j.cell.2022.05.006

実験手法

ELECTRON MICROSCOPY (3.5 Å)