7PV7

Crystal structure of dimeric Porphyromonas gingivalis PorX, a type 9 secretion system response regulator.

7PV7 の概要

• エントリーDOI: 10.2210/pdb7pv7/pdb
• 分子名称: Response regulator, BERYLLIUM TRIFLUORIDE ION, MAGNESIUM ION, ... (7 entities in total)
• 機能のキーワード: response regulator, type 9 secretion system, pglz, phosphodiesterase, signaling protein
• 由来する生物種: Porphyromonas gingivalis (strain ATCC BAA-308 / W83)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 251158.39

構造登録者

Schmitz, C.A.,Madej, M.,Potempa, J.,Sola, M. (登録日: 2021-10-01, 公開日: 2022-12-14, 最終更新日: 2024-10-23)

主引用文献

Schmitz, C.,Madej, M.,Nowakowska, Z.,Cuppari, A.,Jacula, A.,Ksiazek, M.,Mikruta, K.,Wisniewski, J.,Pudelko-Malik, N.,Saran, A.,Zeytuni, N.,Mlynarz, P.,Lamont, R.J.,Uson, I.,Siksnys, V.,Potempa, J.,Sola, M.
Response regulator PorX coordinates oligonucleotide signalling and gene expression to control the secretion of virulence factors.
Nucleic Acids Res., 50:12558-12577, 2022

PubMed Abstract: The PglZ family of proteins belongs to the alkaline phosphatase superfamily, which consists of metallohydrolases with limited sequence identity but similar metal-coordination architectures in otherwise divergent active sites. Proteins with a well-defined PglZ domain are ubiquitous among prokaryotes as essential components of BREX phage defence systems and two-component systems (TCSs). Whereas other members of the alkaline phosphatase superfamily are well characterized, the activity, structure and biological function of PglZ family proteins remain unclear. We therefore investigated the structure and function of PorX, an orphan response regulator of the Porphyromonas gingivalis TCS containing a putative PglZ effector domain. The crystal structure of PorX revealed a canonical receiver domain, a helical bundle, and an unprecedented PglZ domain, similar to the general organization of the phylogenetically related BREX-PglZ proteins. The PglZ domain of PorX features an active site cleft suitable for large substrates. An extensive search for substrates revealed that PorX is a phosphodiesterase that acts on cyclic and linear oligonucleotides, including signalling molecules such as cyclic oligoadenylates. These results, combined with mutagenesis, biophysical and enzymatic analysis, suggest that PorX coordinates oligonucleotide signalling pathways and indirectly regulates gene expression to control the secretion of virulence factors.

PubMed: 36464236
DOI: 10.1093/nar/gkac1103

実験手法

X-RAY DIFFRACTION (2.41 Å)