7PUK

Crystal structure of Endoglycosidase E GH18 domain from Enterococcus faecalis in complex with Man5 product

Summary for 7PUK

• Entry DOI: 10.2210/pdb7puk/pdb
• Descriptor: Beta-N-acetylhexosaminidase, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total)
• Functional Keywords: glycoside hydrolase, gh18, glycan, antibody, enterococcus faecalis, endoe, hydrolase
• Biological source: Enterococcus faecalis (Streptococcus faecalis)
• Total number of polymer chains: 2
• Total formula weight: 99988.50

Authors

Garcia-Alija, M.,Du, J.J.,Trastoy, B.,Sundberg, E.J.,Guerin, M. (deposition date: 2021-09-30, release date: 2022-03-16, Last modification date: 2024-01-31)

Primary citation

Garcia-Alija, M.,Du, J.J.,Ordonez, I.,Diz-Vallenilla, A.,Moraleda-Montoya, A.,Sultana, N.,Huynh, C.G.,Li, C.,Donahue, T.C.,Wang, L.X.,Trastoy, B.,Sundberg, E.J.,Guerin, M.E.
Mechanism of cooperative N-glycan processing by the multi-modular endoglycosidase EndoE.
Nat Commun, 13:1137-1137, 2022

PubMed Abstract: Bacteria produce a remarkably diverse range of glycoside hydrolases to metabolize glycans from the environment as a primary source of nutrients, and to promote the colonization and infection of a host. Here we focus on EndoE, a multi-modular glycoside hydrolase secreted by Enterococcus faecalis, one of the leading causes of healthcare-associated infections. We provide X-ray crystal structures of EndoE, which show an architecture composed of four domains, including GH18 and GH20 glycoside hydrolases connected by two consecutive three α-helical bundles. We determine that the GH20 domain is an exo-β-1,2-N-acetylglucosaminidase, whereas the GH18 domain is an endo-β-1,4-N-acetylglucosaminidase that exclusively processes the central core of complex-type or high-mannose-type N-glycans. Both glycoside hydrolase domains act in a concerted manner to process diverse N-glycans on glycoproteins, including therapeutic IgG antibodies. EndoE combines two enzyme domains with distinct functions and glycan specificities to play a dual role in glycan metabolism and immune evasion.

PubMed: 35241669
DOI: 10.1038/s41467-022-28722-w

Experimental method

X-RAY DIFFRACTION (2.69 Å)