7PUB

Late assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit

これはPDB形式変換不可エントリーです。

7PUB の概要

• エントリーDOI: 10.2210/pdb7pub/pdb
• EMDBエントリー: 13661
• 分子名称: 9S rRNA, uS14m, uS15m, ... (83 entities in total)
• 機能のキーワード: mitoribosome, assembly intermediate, trypanosoma brucei, small subunit, mt-ssu, ssu, ribosome
• 由来する生物種: Trypanosoma brucei brucei; 詳細
• タンパク質・核酸の鎖数: 76
• 化学式量合計: 3617428.02

構造登録者

Lenarcic, T.,Leibundgut, M.,Saurer, M.,Ramrath, D.J.F.,Fluegel, T.,Boehringer, D.,Ban, N. (登録日: 2021-09-29, 公開日: 2022-05-04, 最終更新日: 2024-11-20)

主引用文献

Lenarcic, T.,Niemann, M.,Ramrath, D.J.F.,Calderaro, S.,Flugel, T.,Saurer, M.,Leibundgut, M.,Boehringer, D.,Prange, C.,Horn, E.K.,Schneider, A.,Ban, N.
Mitoribosomal small subunit maturation involves formation of initiation-like complexes.
Proc.Natl.Acad.Sci.USA, 119:-, 2022

PubMed Abstract: Mitochondrial ribosomes (mitoribosomes) play a central role in synthesizing mitochondrial inner membrane proteins responsible for oxidative phosphorylation. Although mitoribosomes from different organisms exhibit considerable structural variations, recent insights into mitoribosome assembly suggest that mitoribosome maturation follows common principles and involves a number of conserved assembly factors. To investigate the steps involved in the assembly of the mitoribosomal small subunit (mt-SSU) we determined the cryoelectron microscopy structures of middle and late assembly intermediates of the mitochondrial small subunit (mt-SSU) at 3.6- and 3.7-Å resolution, respectively. We identified five additional assembly factors that together with the mitochondrial initiation factor 2 (mt-IF-2) specifically interact with functionally important regions of the rRNA, including the decoding center, thereby preventing premature mRNA or large subunit binding. Structural comparison of assembly intermediates with mature mt-SSU combined with RNAi experiments suggests a noncanonical role of mt-IF-2 and a stepwise assembly process, where modular exchange of ribosomal proteins and assembly factors together with mt-IF-2 ensure proper 9S rRNA folding and protein maturation during the final steps of assembly.

PubMed: 35042777
DOI: 10.1073/pnas.2114710118

実験手法

ELECTRON MICROSCOPY (3.7 Å)