7PRN

E1M, D97A, E99A MUTANT OF RH. BLASTICA PORIN

7PRN の概要

• エントリーDOI: 10.2210/pdb7prn/pdb
• 分子名称: PORIN, (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE (3 entities in total)
• 機能のキーワード: integral membrane protein, porin, pore eyelet mutant, membrane protein
• 由来する生物種: Rhodobacter blasticus
• 細胞内の位置: Cell outer membrane; Multi-pass membrane protein: P39767
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30815.33

構造登録者

Maveyraud, L.,Schmid, B.,Schulz, G.E. (登録日: 1998-06-12, 公開日: 1998-08-12, 最終更新日: 2024-05-22)

主引用文献

Schmid, B.,Maveyraud, L.,Kromer, M.,Schulz, G.E.
Porin mutants with new channel properties.
Protein Sci., 7:1603-1611, 1998

PubMed Abstract: The general diffusion porin from Rhodopseudomonas blastica was produced in large amounts in Escherichia coli inclusion bodies and (re)natured to the exact native structure. Here, we report on 13 mutants at the pore eyelet giving rise to new diffusion properties as measured in planar lipid bilayer experiments. The crystal structures of seven of these mutants were established. The effects of charge-modifying mutations at the pore eyelet are consistent with the known selectivity for cations. Deletions of 16 and 27 residues of the constriction loop L3 resulted in labile trimers and pores. The reduction of the eyelet cross section by introducing tryptophans gave rise to a closely correlated decrease of the conductivities. A mutant with six newly introduced tryptophans in the eyelet closed its pore in a defined manner within seconds under a voltage of 20 mV, suggesting the existence of two states. The results indicate that the pore can be engineered in a rational manner.

PubMed: 9684893

実験手法

X-RAY DIFFRACTION (2.25 Å)