7PRM

CRYSTAL STRUCTURE OF HUMAN MONOGLYCERIDE LIPASE WITH COMPOUND 13

7PRM の概要

• エントリーDOI: 10.2210/pdb7prm/pdb
• 分子名称: Monoglyceride lipase, (4~{R})-1-[4-(4-fluorophenyl)phenyl]-4-[4-(furan-2-ylcarbonyl)piperazin-1-yl]pyrrolidin-2-one, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: alpha/beta hydrolase, hydrolase, serine esterase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36023.12

構造登録者

Grether, U.,Gobbi, L.,Kuhn, B.,Collin, L.,Leibrock, L.,Heer, D.,Wittwer, M.,Benz, J. (登録日: 2021-09-22, 公開日: 2022-02-16, 最終更新日: 2024-05-01)

主引用文献

He, Y.,Schild, M.,Grether, U.,Benz, J.,Leibrock, L.,Heer, D.,Topp, A.,Collin, L.,Kuhn, B.,Wittwer, M.,Keller, C.,Gobbi, L.C.,Schibli, R.,Mu, L.
Development of High Brain-Penetrant and Reversible Monoacylglycerol Lipase PET Tracers for Neuroimaging.
J.Med.Chem., 65:2191-2207, 2022

PubMed Abstract: Monoacylglycerol lipase (MAGL) is one of the key enzymes in the endocannabinoid system. Inhibition of MAGL has been proposed as an attractive approach for the treatment of various diseases. In this study, we designed and successfully synthesized two series of piperazinyl pyrrolidin-2-one derivatives as novel reversible MAGL inhibitors. ()-[F] was identified through the preliminary evaluation of two carbon-11-labeled racemic structures [C] and [C]. In dynamic positron-emission tomography (PET) scans, ()-[F] showed a heterogeneous distribution and matched the MAGL expression pattern in the mouse brain. High brain uptake and brain-to-blood ratio were achieved by ()-[F] in comparison with previously reported reversible MAGL PET radiotracers. Target occupancy studies with a therapeutic MAGL inhibitor revealed a dose-dependent reduction of ()-[F] accumulation in the mouse brain. These findings indicate that ()-[F] ([F]YH149) is a highly promising PET probe for visualizing MAGL non-invasively and holds great potential to support drug development.

PubMed: 35089028
DOI: 10.1021/acs.jmedchem.1c01706

実験手法

X-RAY DIFFRACTION (1.65 Å)