7PR0

Crystal structure of the receptor binding domain of SARS-CoV-2 Spike glycoprotein in complex with FD-5D Fab

7PR0 の概要

• エントリーDOI: 10.2210/pdb7pr0/pdb
• 関連するPDBエントリー: 7PQY 7PQZ
• 分子名称: Spike protein S1, FD-5D Fab light chain, FD-5D Fab heavy chain, ... (8 entities in total)
• 機能のキーワード: sars-cov-2 antibody, receptor-binding-domain, rbd, spike, neutralisation, fi-3a, fd-11a, fd-5d, viral protein/immune system, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 217060.03

構造登録者

Zhou, D.,Ren, J.,Stuart, D.I. (登録日: 2021-09-20, 公開日: 2022-02-02, 最終更新日: 2024-11-20)

主引用文献

Huang, K.A.,Zhou, D.,Tan, T.K.,Chen, C.,Duyvesteyn, H.M.E.,Zhao, Y.,Ginn, H.M.,Qin, L.,Rijal, P.,Schimanski, L.,Donat, R.,Harding, A.,Gilbert-Jaramillo, J.,James, W.,Tree, J.A.,Buttigieg, K.,Carroll, M.,Charlton, S.,Lien, C.E.,Lin, M.Y.,Chen, C.P.,Cheng, S.H.,Chen, X.,Lin, T.Y.,Fry, E.E.,Ren, J.,Ma, C.,Townsend, A.R.,Stuart, D.I.
Structures and therapeutic potential of anti-RBD human monoclonal antibodies against SARS-CoV-2.
Theranostics, 12:1-17, 2022

PubMed Abstract: Administration of potent anti-receptor-binding domain (RBD) monoclonal antibodies has been shown to curtail viral shedding and reduce hospitalization in patients with SARS-CoV-2 infection. However, the structure-function analysis of potent human anti-RBD monoclonal antibodies and its links to the formulation of antibody cocktails remains largely elusive. Previously, we isolated a panel of neutralizing anti-RBD monoclonal antibodies from convalescent patients and showed their neutralization efficacy . Here, we elucidate the mechanism of action of antibodies and dissect antibodies at the epitope level, which leads to a formation of a potent antibody cocktail. We found that representative antibodies which target non-overlapping epitopes are effective against wild type virus and recently emerging variants of concern, whilst being encoded by antibody genes with few somatic mutations. Neutralization is associated with the inhibition of binding of viral RBD to ACE2 and possibly of the subsequent fusion process. Structural analysis of representative antibodies, by cryo-electron microscopy and crystallography, reveals that they have some unique aspects that are of potential value while sharing some features in common with previously reported neutralizing monoclonal antibodies. For instance, one has a common VH 3-53 public variable region yet is unusually resilient to mutation at residue 501 of the RBD. We evaluate the efficacy of an antibody cocktail consisting of two potent non-competing anti-RBD antibodies in a Syrian hamster model. We demonstrate that the cocktail prevents weight loss, reduces lung viral load and attenuates pulmonary inflammation in hamsters in both prophylactic and therapeutic settings. Although neutralization of one of these antibodies is abrogated by the mutations of variant B.1.351, it is also possible to produce a bi-valent cocktail of antibodies both of which are resilient to variants B.1.1.7, B.1.351 and B.1.617.2. These findings support the up-to-date and rational design of an anti-RBD antibody cocktail as a therapeutic candidate against COVID-19.

PubMed: 34987630
DOI: 10.7150/thno.65563

実験手法

X-RAY DIFFRACTION (2.92 Å)