7PNT

Assembly intermediate of mouse mitochondrial ribosome small subunit without mS37 in complex with RbfA and Tfb1m

これはPDB形式変換不可エントリーです。

7PNT の概要

• エントリーDOI: 10.2210/pdb7pnt/pdb
• EMDBエントリー: 13551
• 分子名称: 12S mitochondrial rRNA, 28S ribosomal protein S12, mitochondrial, 28S ribosomal protein S14, mitochondrial, ... (38 entities in total)
• 機能のキーワード: assembly intermediate, maturation, biogenesis, small subunit, mitochondrion, ribosome
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 32
• 化学式量合計: 1195134.67

構造登録者

Itoh, Y.,Khawaja, A.,Laptev, I.,Sergiev, P.,Rorbach, J.,Amunts, A. (登録日: 2021-09-08, 公開日: 2022-06-15, 最終更新日: 2024-01-17)

主引用文献

Itoh, Y.,Khawaja, A.,Laptev, I.,Cipullo, M.,Atanassov, I.,Sergiev, P.,Rorbach, J.,Amunts, A.
Mechanism of mitoribosomal small subunit biogenesis and preinitiation.
Nature, 606:603-608, 2022

PubMed Abstract: Mitoribosomes are essential for the synthesis and maintenance of bioenergetic proteins. Here we use cryo-electron microscopy to determine a series of the small mitoribosomal subunit (SSU) intermediates in complex with auxiliary factors, revealing a sequential assembly mechanism. The methyltransferase TFB1M binds to partially unfolded rRNA h45 that is promoted by RBFA, while the mRNA channel is blocked. This enables binding of METTL15 that promotes further rRNA maturation and a large conformational change of RBFA. The new conformation allows initiation factor mtIF3 to already occupy the subunit interface during the assembly. Finally, the mitochondria-specific ribosomal protein mS37 (ref. ) outcompetes RBFA to complete the assembly with the SSU-mS37-mtIF3 complex that proceeds towards mtIF2 binding and translation initiation. Our results explain how the action of step-specific factors modulate the dynamic assembly of the SSU, and adaptation of a unique protein, mS37, links the assembly to initiation to establish the catalytic human mitoribosome.

PubMed: 35676484
DOI: 10.1038/s41586-022-04795-x

実験手法

ELECTRON MICROSCOPY (3.19 Å)