7PKT

Large subunit of the Chlamydomonas reinhardtii mitoribosome

これはPDB形式変換不可エントリーです。

7PKT の概要

• エントリーDOI: 10.2210/pdb7pkt/pdb
• 関連するPDBエントリー: 7PKQ
• EMDBエントリー: 13477 13480 13481 13578
• 分子名称: Ribosomal_L2_C domain-containing protein, Mitochondrial ribosomal protein L17,bL17m, Mitochondrial ribosomal protein L19, ... (57 entities in total)
• 機能のキーワード: mitochondria, mitoribosome, alga, ribosome
• 由来する生物種: Chlamydomonas reinhardtii; 詳細
• タンパク質・核酸の鎖数: 54
• 化学式量合計: 1926381.06

構造登録者

Waltz, F.,Soufari, H.,Hashem, Y. (登録日: 2021-08-26, 公開日: 2022-06-15, 最終更新日: 2024-07-17)

主引用文献

Waltz, F.,Salinas-Giege, T.,Englmeier, R.,Meichel, H.,Soufari, H.,Kuhn, L.,Pfeffer, S.,Forster, F.,Engel, B.D.,Giege, P.,Drouard, L.,Hashem, Y.
How to build a ribosome from RNA fragments in Chlamydomonas mitochondria.
Nat Commun, 12:7176-7176, 2021

PubMed Abstract: Mitochondria are the powerhouse of eukaryotic cells. They possess their own gene expression machineries where highly divergent and specialized ribosomes, named hereafter mitoribosomes, translate the few essential messenger RNAs still encoded by mitochondrial genomes. Here, we present a biochemical and structural characterization of the mitoribosome in the model green alga Chlamydomonas reinhardtii, as well as a functional study of some of its specific components. Single particle cryo-electron microscopy resolves how the Chlamydomonas mitoribosome is assembled from 13 rRNA fragments encoded by separate non-contiguous gene pieces. Additional proteins, mainly OPR, PPR and mTERF helical repeat proteins, are found in Chlamydomonas mitoribosome, revealing the structure of an OPR protein in complex with its RNA binding partner. Targeted amiRNA silencing indicates that these ribosomal proteins are required for mitoribosome integrity. Finally, we use cryo-electron tomography to show that Chlamydomonas mitoribosomes are attached to the inner mitochondrial membrane via two contact points mediated by Chlamydomonas-specific proteins. Our study expands our understanding of mitoribosome diversity and the various strategies these specialized molecular machines adopt for membrane tethering.

PubMed: 34887394
DOI: 10.1038/s41467-021-27200-z

実験手法

ELECTRON MICROSCOPY (3 Å)