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- EMDB-13480: Large subunit of the Chlamydomonas reinhardtii mitoribosome -

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Basic information

Entry
Database: EMDB / ID: EMD-13480
TitleLarge subunit of the Chlamydomonas reinhardtii mitoribosome
Map dataLarge subunit of the Chlamydomonas mitoribosome
Sample
  • Complex: Large subunit of the mitochondrial ribosome of Chlamydomonas reinhardtii
    • Protein or peptide: x 45 types
    • RNA: x 9 types
  • Ligand: x 3 types
KeywordsMitochondria / mitoribosome / alga / RIBOSOME
Function / homology
Function and homology information


chloroplast rRNA processing / regulation of mitochondrial mRNA stability / ribonucleoprotein granule / mitochondrial RNA processing / mitochondrial large ribosomal subunit / mitochondrial ribosome / mitochondrial translation / chloroplast / large ribosomal subunit / 5S rRNA binding ...chloroplast rRNA processing / regulation of mitochondrial mRNA stability / ribonucleoprotein granule / mitochondrial RNA processing / mitochondrial large ribosomal subunit / mitochondrial ribosome / mitochondrial translation / chloroplast / large ribosomal subunit / 5S rRNA binding / large ribosomal subunit rRNA binding / transferase activity / cytosolic large ribosomal subunit / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / mitochondrial matrix / ribonucleoprotein complex / translation / mRNA binding / mitochondrion / RNA binding / cytoplasm
Similarity search - Function
: / Growth arrest and DNA damage-inducible proteins-interacting protein 1 domain superfamily / 39S ribosomal protein L46, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain ...: / Growth arrest and DNA damage-inducible proteins-interacting protein 1 domain superfamily / 39S ribosomal protein L46, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / NUDIX hydrolase-like domain superfamily / : / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L17 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / : / Ribosomal protein L6, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / : / L28p-like / Ribosomal protein L20 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal L32p protein family / Ribosomal protein L32p / Ribosomal protein L24 / Ribosomal protein L13, bacterial-type / Ribosomal protein L3, bacterial/organelle-type / Ribosomal protein L15, bacterial-type / 50S ribosomal protein uL4 / Ribosomal protein L34 / Ribosomal protein L34 / Ribosomal protein L2 signature. / Ribosomal protein L2, conserved site / Ribosomal protein L5 domain superfamily / : / Ribosomal protein L10e/L16 / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L16p/L10e / Ribosomal protein L6, alpha-beta domain / Ribosomal protein L6 / Ribosomal protein L6 / Ribosomal protein L6, alpha-beta domain superfamily / Ribosomal protein L2, domain 3 / Ribosomal protein L14P, conserved site / Ribosomal protein L14 signature. / Ribosomal Proteins L2, C-terminal domain / Ribosomal protein L2, C-terminal / Ribosomal Proteins L2, C-terminal domain / Ribosomal protein L24 signature. / Ribosomal Proteins L2, RNA binding domain / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein L24/L26, conserved site / Ribosomal Proteins L2, RNA binding domain / KOW (Kyprides, Ouzounis, Woese) motif. / Ribosomal protein L2 / Ribosomal protein L13 / Ribosomal protein L13 / Ribosomal protein L13 superfamily / Ribosomal protein L23 / Ribosomal protein L30, ferredoxin-like fold domain / Ribosomal protein L30p/L7e / Ribosomal protein L15 / Ribosomal protein L25/L23 / Prokaryotic membrane lipoprotein lipid attachment site profile. / Ribosomal protein L30, ferredoxin-like fold domain superfamily
Similarity search - Domain/homology
Large ribosomal subunit protein bL34m / Uncharacterized protein / Mitochondrial ribosomal protein L28 / Large ribosomal subunit protein uL4m / Flagellar associated protein / Large ribosomal subunit protein uL15/eL18 domain-containing protein / Uncharacterized protein / Uncharacterized protein / Large ribosomal subunit protein bL21m / KOW domain-containing protein ...Large ribosomal subunit protein bL34m / Uncharacterized protein / Mitochondrial ribosomal protein L28 / Large ribosomal subunit protein uL4m / Flagellar associated protein / Large ribosomal subunit protein uL15/eL18 domain-containing protein / Uncharacterized protein / Uncharacterized protein / Large ribosomal subunit protein bL21m / KOW domain-containing protein / Uncharacterized protein / Large ribosomal subunit protein uL2 C-terminal domain-containing protein / Uncharacterized protein / Uncharacterized protein / Large ribosomal subunit protein bL9c / Large ribosomal subunit protein uL22m / Large ribosomal subunit protein uL30m / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Mitochondrial ribosomal protein L17 / Uncharacterized protein / Large ribosomal subunit protein bL25 beta domain-containing protein / Mitochondrial ribosomal protein L3 / Ribosomal protein L27 / 50S ribosomal protein L20 / Predicted protein / Predicted protein / Predicted protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 50S ribosomal protein L14 / Uncharacterized protein / Uncharacterized protein / Large ribosomal subunit protein uL6 alpha-beta domain-containing protein / Uncharacterized protein / Mitochondrial ribosomal protein L23 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Mitochondrial ribosomal protein L17
Similarity search - Component
Biological speciesChlamydomonas reinhardtii (plant)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.0 Å
AuthorsWaltz F / Soufari H / Hashem Y
Funding supportEuropean Union, France, 4 items
OrganizationGrant numberCountry
European Research Council (ERC)TransTryp ID:759120European Union
Agence Nationale de la Recherche (ANR)ANR-11-LABX-0057_MITOCROSS France
Agence Nationale de la Recherche (ANR)MITRA, ANR-16-CE11-0024-02 France
Agence Nationale de la Recherche (ANR)DAMIA, ANR-20-CE11-0021 France
CitationJournal: Nat Commun / Year: 2021
Title: How to build a ribosome from RNA fragments in Chlamydomonas mitochondria.
Authors: Florent Waltz / Thalia Salinas-Giegé / Robert Englmeier / Herrade Meichel / Heddy Soufari / Lauriane Kuhn / Stefan Pfeffer / Friedrich Förster / Benjamin D Engel / Philippe Giegé / ...Authors: Florent Waltz / Thalia Salinas-Giegé / Robert Englmeier / Herrade Meichel / Heddy Soufari / Lauriane Kuhn / Stefan Pfeffer / Friedrich Förster / Benjamin D Engel / Philippe Giegé / Laurence Drouard / Yaser Hashem /
Abstract: Mitochondria are the powerhouse of eukaryotic cells. They possess their own gene expression machineries where highly divergent and specialized ribosomes, named hereafter mitoribosomes, translate the ...Mitochondria are the powerhouse of eukaryotic cells. They possess their own gene expression machineries where highly divergent and specialized ribosomes, named hereafter mitoribosomes, translate the few essential messenger RNAs still encoded by mitochondrial genomes. Here, we present a biochemical and structural characterization of the mitoribosome in the model green alga Chlamydomonas reinhardtii, as well as a functional study of some of its specific components. Single particle cryo-electron microscopy resolves how the Chlamydomonas mitoribosome is assembled from 13 rRNA fragments encoded by separate non-contiguous gene pieces. Additional proteins, mainly OPR, PPR and mTERF helical repeat proteins, are found in Chlamydomonas mitoribosome, revealing the structure of an OPR protein in complex with its RNA binding partner. Targeted amiRNA silencing indicates that these ribosomal proteins are required for mitoribosome integrity. Finally, we use cryo-electron tomography to show that Chlamydomonas mitoribosomes are attached to the inner mitochondrial membrane via two contact points mediated by Chlamydomonas-specific proteins. Our study expands our understanding of mitoribosome diversity and the various strategies these specialized molecular machines adopt for membrane tethering.
History
DepositionAug 26, 2021-
Header (metadata) releaseJun 15, 2022-
Map releaseJun 15, 2022-
UpdateJul 17, 2024-
Current statusJul 17, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_13480.png

Downloads & links

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Map

FileDownload / File: emd_13480.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationLarge subunit of the Chlamydomonas mitoribosome
Projections & slices

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AxesZ (Sec.)Y (Row.)X (Col.)
0.9 Å/pix.
x 480 pix.
= 432. Å		0.9 Å/pix.
x 480 pix.
= 432. Å		0.9 Å/pix.
x 480 pix.
= 432. Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.9 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.014
Minimum - Maximum-0.045484774 - 0.09165091
Average (Standard dev.)-0.000046097455 (±0.003166966)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 432.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_13480_half_map_1.map
Projections & Slices
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Half map: #2

Fileemd_13480_half_map_2.map
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Sample components

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Entire : Large subunit of the mitochondrial ribosome of Chlamydomonas rein...

EntireName: Large subunit of the mitochondrial ribosome of Chlamydomonas reinhardtii
Components
  • Complex: Large subunit of the mitochondrial ribosome of Chlamydomonas reinhardtii
    • Protein or peptide: Ribosomal_L2_C domain-containing protein
    • Protein or peptide: uL3m
    • Protein or peptide: uL4m
    • Protein or peptide: uL5m
    • Protein or peptide: 50S ribosomal protein L9, chloroplastic
    • Protein or peptide: Mitochondrial ribosomal protein L13
    • Protein or peptide: uL14m
    • Protein or peptide: Ribosomal_L18e/L15P domain-containing protein
    • Protein or peptide: Ribosomal_L16 domain-containing protein
    • Protein or peptide: Mitochondrial ribosomal protein L17,bL17m
    • Protein or peptide: Mitochondrial ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: bL21m
    • Protein or peptide: uL22m
    • Protein or peptide: Mitochondrial ribosomal protein L23
    • Protein or peptide: KOW domain-containing protein,uL24m
    • Protein or peptide: Ribosomal_TL5_C domain-containing protein
    • Protein or peptide: bL27m
    • Protein or peptide: bL28m
    • Protein or peptide: uL29m
    • Protein or peptide: Ribosomal_L30 domain-containing protein
    • Protein or peptide: bL32m
    • Protein or peptide: Mitochondrial ribosomal protein L33
    • Protein or peptide: bL34m
    • Protein or peptide: bL35m
    • Protein or peptide: Plastid ribosomal protein L6
    • Protein or peptide: mL40
    • Protein or peptide: mL41
    • Protein or peptide: mL43
    • Protein or peptide: Mitochondrial ribosomal protein L17
    • Protein or peptide: mL63/57/60
    • Protein or peptide: mL59/64
    • Protein or peptide: mL80
    • Protein or peptide: mL87
    • Protein or peptide: bL36m
    • Protein or peptide: mL113
    • Protein or peptide: mL114
    • Protein or peptide: mL115
    • Protein or peptide: mL116
    • Protein or peptide: mL117
    • Protein or peptide: mL118
    • Protein or peptide: mL119
    • Protein or peptide: Unk1
    • Protein or peptide: PPR*
    • Protein or peptide: Unk2
    • RNA: L1 rRNA
    • RNA: L2a rRNA
    • RNA: L3a rRNA (5S)
    • RNA: L3b rRNA
    • RNA: L4 rRNA
    • RNA: L5 rRNA
    • RNA: L6 rRNA
    • RNA: L7 rRNA
    • RNA: L8 rRNA
  • Ligand: ZINC ION
  • Ligand: MAGNESIUM ION
  • Ligand: FE2/S2 (INORGANIC) CLUSTER

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Supramolecule #1: Large subunit of the mitochondrial ribosome of Chlamydomonas rein...

SupramoleculeName: Large subunit of the mitochondrial ribosome of Chlamydomonas reinhardtii
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#54
Source (natural)Organism: Chlamydomonas reinhardtii (plant) / Organelle: Mitochondria

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Macromolecule #1: Ribosomal_L2_C domain-containing protein

MacromoleculeName: Ribosomal_L2_C domain-containing protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 41.087098 KDa
SequenceString: MLRRVAQSCA TMLSKQGAGA MDMAQPSTSA PMFNLANEAL QAAGSGHGPA GTLLQFIRSK YTYKFKKAAP LPGLIYWKPS TPGTRHKIS IDYAGLGVYT GPPHPELSAH VAKTGGRNNT GRITTRHRGG GAPKVVRDVD FDRQRVDGLP GVVQRLEADP G RSGWLALV ...String:
MLRRVAQSCA TMLSKQGAGA MDMAQPSTSA PMFNLANEAL QAAGSGHGPA GTLLQFIRSK YTYKFKKAAP LPGLIYWKPS TPGTRHKIS IDYAGLGVYT GPPHPELSAH VAKTGGRNNT GRITTRHRGG GAPKVVRDVD FDRQRVDGLP GVVQRLEADP G RSGWLALV KYSPEGQLPF YRYHLAPADV KAGDVLMSGH GAAIKPGNVL TLRDVPIGVP IHNIELVPGR GGQLARAAST SA VVTSKQD THAVVRLPSS ETRLIDLSCR ATVGQVSNHL QRSINHGKAG SRRNLGWRPS VRGIAMNPID HPHGGRTNGG RPS CTPWGV YTKGKRTRRR DASSNKFIIT RAGGQPIEKF VQAKKWRARA KLEAKQRAAA GGGGKRS

UniProtKB: Large ribosomal subunit protein uL2 C-terminal domain-containing protein

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Macromolecule #2: uL3m

MacromoleculeName: uL3m / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 44.936207 KDa
SequenceString: MSATGSSCAL PTGIETSGGL PLQENSGDLA AASSAEASDA CRAASHGLAS TSLRCLATRE NHSFPDGWGT LCSQGLAASG LQLQQRRGY GYQPPKPRFP LPDSIASIVE EKTREHRRSA PAPPPAPKLE PRPMTPTSLR TGCIATKAGM TQEWDEHGVR V PLTVLWVD ...String:
MSATGSSCAL PTGIETSGGL PLQENSGDLA AASSAEASDA CRAASHGLAS TSLRCLATRE NHSFPDGWGT LCSQGLAASG LQLQQRRGY GYQPPKPRFP LPDSIASIVE EKTREHRRSA PAPPPAPKLE PRPMTPTSLR TGCIATKAGM TQEWDEHGVR V PLTVLWVD ECQVVGLKTR PVHGYNALML GSGYKRQKCM SPSEAGFFLK AGVPFKKLVA EWQVSEDALV PVGTAIGAAH YV AGQRVDV TGWTKWKGFQ GVMRRWGFKG LPASHGVSLS HRAPGSIGNR QDPGKIWKGK KLPGCMGDER RTVHNCLVYK VDA ARNLVY LRGQVPGPVG RSVFLRDSRL ASPALRASWG LPFPTHVPSA EELKAAPAPG DVSVVPAPDG PGVTAWRNPT DPYL MYREE TDYMPVKWKK GE

UniProtKB: Mitochondrial ribosomal protein L3

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Macromolecule #3: uL4m

MacromoleculeName: uL4m / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 45.910914 KDa
SequenceString: MLAGALRGCA SESAFAWRQV VSAAAAAGSC SGSAGRLMVS SPCGVAQTSS PLQRWLFQGL RSSSTGAASI SGGLSEAGSL PPLVLRRVD DEALSPHPPT TDVGPLTVRY PFPIEYYKDR EAVIYSLDER PLGLAPLPGA AFNVPVRIDI LHRVVRYWRA K WQQGTHKA ...String:
MLAGALRGCA SESAFAWRQV VSAAAAAGSC SGSAGRLMVS SPCGVAQTSS PLQRWLFQGL RSSSTGAASI SGGLSEAGSL PPLVLRRVD DEALSPHPPT TDVGPLTVRY PFPIEYYKDR EAVIYSLDER PLGLAPLPGA AFNVPVRIDI LHRVVRYWRA K WQQGTHKA KSRAEVSGGG KKPWNQKKTG RARQGSIRSP LWKGGGVSHA PRPRSHAHAL PRSTRLLGMR CALSAKINEG RF FVVDDLI NLRAAPLQ(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)SNKNP ARWSRHGLSP ADRPIREYGE LKRRLGALTE GSFGSSWLLV DSGEA GRDG GLRLRKLLKC SVVMEVVSPE ELTVYHVLKY HRLVVTRDAL QRISEALTRP HRVTKPVKHA WWARRRQAID AAVQEL TQA EAQA

UniProtKB: Large ribosomal subunit protein uL4m, Large ribosomal subunit protein uL4m

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Macromolecule #4: uL5m

MacromoleculeName: uL5m / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 23.054836 KDa
SequenceString: MLKLQPRSWD ALPRLTAIEV SIPAIETQLE RDVVDKSELL LYALALEVLA GKPAGFTAPA NKALGTRATG VAVRLDAVTE PEAAHLFME KLVHVLLPNQ VGFEGVPPPM LVPPPRRSKA AEAAQARKAA LDHRKAPAKA HFTEIKVGNL LTYPDFEQNF S LFEPLRGM ...String:
MLKLQPRSWD ALPRLTAIEV SIPAIETQLE RDVVDKSELL LYALALEVLA GKPAGFTAPA NKALGTRATG VAVRLDAVTE PEAAHLFME KLVHVLLPNQ VGFEGVPPPM LVPPPRRSKA AEAAQARKAA LDHRKAPAKA HFTEIKVGNL LTYPDFEQNF S LFEPLRGM RVRLVMEGAS AADCAALLGG MSLPVLSGAA AEAALAEITA EVARRARG

UniProtKB: Uncharacterized protein

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Macromolecule #5: 50S ribosomal protein L9, chloroplastic

MacromoleculeName: 50S ribosomal protein L9, chloroplastic / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 30.622402 KDa
SequenceString: MQTAVAIGAR AGRGALLRSL LPCISGTIES ATSSQEAVER SGGLASTSST PAHGPSCACA RCFTSLAFST TRHASTAAAA KAKGKSSKS SAGRQAGTEA ADLDPGLIRV VLRKDVPRLG RRGEVVSVRR GLMRHSLYPA GEAAYATPDN IAKYGLADVE R GMDAAAEQ ...String:
MQTAVAIGAR AGRGALLRSL LPCISGTIES ATSSQEAVER SGGLASTSST PAHGPSCACA RCFTSLAFST TRHASTAAAA KAKGKSSKS SAGRQAGTEA ADLDPGLIRV VLRKDVPRLG RRGEVVSVRR GLMRHSLYPA GEAAYATPDN IAKYGLADVE R GMDAAAEQ QQGGLEKLIK ALDTKPVVIT RRASDSDPES FAPGSAAVGA RAVAAAVESQ LGIRLDAASH LFLGEPIRSY GS YKVPLNL RLASEPQRQV ELTVHVDRRG KPQQPGATAA AGGGSSGEGG AGAAAGAAGG AGAAASS

UniProtKB: Large ribosomal subunit protein bL9c

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Macromolecule #6: Mitochondrial ribosomal protein L13

MacromoleculeName: Mitochondrial ribosomal protein L13 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 29.714832 KDa
SequenceString: MSRVTEAIKH LKDLQHIKLE GLRFRIIDAK GQVVGRLAEQ ISVILQGKDK PTYNPTKEQG DVVIVVNASH VELTHDKWNT KLYRWHTGY PGGLRSRPAG DQWEKDPRML LRNAVSGMLP KNKSREARME KLKIFPEADH PFQGFPLVPY VPPPRRLQDR G LGWPLPQG ...String:
MSRVTEAIKH LKDLQHIKLE GLRFRIIDAK GQVVGRLAEQ ISVILQGKDK PTYNPTKEQG DVVIVVNASH VELTHDKWNT KLYRWHTGY PGGLRSRPAG DQWEKDPRML LRNAVSGMLP KNKSREARME KLKIFPEADH PFQGFPLVPY VPPPRRLQDR G LGWPLPQG FEPANLERYA FRLRTSPALQ AAAAPTVPAS SALAAAPQPS APVTGPGPAA SAPAAAAAAA GTAGASGSGS GR QQRQRPT VPIDDLLTEE ERAALAAVEG GKGKSGASSS

UniProtKB: Uncharacterized protein

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Macromolecule #7: uL14m

MacromoleculeName: uL14m / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 13.356652 KDa
SequenceString:
MIPKYSFLKI VDNSGAKLAQ VIGFYGHQGG YASVGDVVKV VVKEARGEKT TAGVMRKAVV VEQKYPFHRQ NGSHVQYLRN AAVLLSEKG QPIGNKVRSM LGVEFNKPRW AKLKSLSKRL F

UniProtKB: 50S ribosomal protein L14

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Macromolecule #8: Ribosomal_L18e/L15P domain-containing protein

MacromoleculeName: Ribosomal_L18e/L15P domain-containing protein / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 35.532332 KDa
SequenceString: MSRSAAAGLL ARLGITCQRS CSVTFQIAST IAATASSAPS CSYTQHSPGA FASAGAPRGL ASLAFWTRRE AAASSSISDT LSSGAQPQQ QHLAASREAQ QARGFAAQAA GPDHLVGLGN LRDNPGATRQ RIRVGRGDGS RRGNYGGRGM KGQKSRGRGL H MLYDGGQL ...String:
MSRSAAAGLL ARLGITCQRS CSVTFQIAST IAATASSAPS CSYTQHSPGA FASAGAPRGL ASLAFWTRRE AAASSSISDT LSSGAQPQQ QHLAASREAQ QARGFAAQAA GPDHLVGLGN LRDNPGATRQ RIRVGRGDGS RRGNYGGRGM KGQKSRGRGL H MLYDGGQL GLLKFPVTRQ RPSYEVLYYH LGLSRVAEFV QLGLLDAGRT ITMKDLYDSG CVTSNIKYGV LLYGKARLAV PL DLQVTAC DADTRAAVES AGGRVTRVYY TAEGLGAVLH PEKFTSRRLP LPLPAPRWHP RYDKKFDAIG QIPPATRPVA AAA LPAAAA HASGRVALAS A

UniProtKB: Large ribosomal subunit protein uL15/eL18 domain-containing protein

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Macromolecule #9: Ribosomal_L16 domain-containing protein

MacromoleculeName: Ribosomal_L16 domain-containing protein / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 27.922502 KDa
SequenceString: MVAEGSSAAT TSCSGRGLVT WTRQGPGASS ALGASLPGSS VPTFARLCPN LSVTAAVQLP GGVLLQRAGL HSLVASRAGS AAGAASAAR SFASPLGPLL APTAGASAAA AGASPGSSSG LLLGGWSACA GPVRWCQAGQ LSKQRVFKRE GINDVIMPNT T QLRYGTYG ...String:
MVAEGSSAAT TSCSGRGLVT WTRQGPGASS ALGASLPGSS VPTFARLCPN LSVTAAVQLP GGVLLQRAGL HSLVASRAGS AAGAASAAR SFASPLGPLL APTAGASAAA AGASPGSSSG LLLGGWSACA GPVRWCQAGQ LSKQRVFKRE GINDVIMPNT T QLRYGTYG IRAMASKRVG AATIEAVRRT LRRKIKKTAR LWIRLSAVVP VTRKPLGIRM GKGKGVIDFY ATPVRPGQII FE MDRVPRK VALQALTAAQ HKFPVKLGFV EWS

UniProtKB: Uncharacterized protein

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Macromolecule #10: Mitochondrial ribosomal protein L17,bL17m

MacromoleculeName: Mitochondrial ribosomal protein L17,bL17m / type: protein_or_peptide / ID: 10
Details: The protein is a mixture of A0A2K3DXS2 and A8JH49,The protein is a mixture of A0A2K3DXS2 and A8JH49
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 21.380727 KDa
SequenceString:
ARVRVRLGDW NRLNRKWQHR VSMMKTMVTQ LLEKERIRTT LAKAKTLQRY ADKVITLGKQ GTREAWSEAR GIVRTDRELH KLFTQVALR YRERPGGFTR LVRAGWRERD AAPVAFVELV DRPGELRPAN PALQRPNPLS VQAWIRQQLG QPAREPSPLL P PSVQQYLR EENRIQQGRA QPVTR

UniProtKB: Mitochondrial ribosomal protein L17, Mitochondrial ribosomal protein L17

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Macromolecule #11: Mitochondrial ribosomal protein L19

MacromoleculeName: Mitochondrial ribosomal protein L19 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 33.412934 KDa
SequenceString: MLGALLASLG ASGPRALSHY SASTSSLALQ SSIESIKETG AAYKALGTGW RFWSAGAGAS ARGDVNASSP AQAKSDESAA YASTAAPHE PAASPLNGDA QLSELLGDGG HPVASASGSS ARSGGGRSPP SGLPRLSLPQ RVLRDLRPPV QPYTLAPKLA Q RPTTTPAQ ...String:
MLGALLASLG ASGPRALSHY SASTSSLALQ SSIESIKETG AAYKALGTGW RFWSAGAGAS ARGDVNASSP AQAKSDESAA YASTAAPHE PAASPLNGDA QLSELLGDGG HPVASASGSS ARSGGGRSPP SGLPRLSLPQ RVLRDLRPPV QPYTLAPKLA Q RPTTTPAQ YTPWTPTRAL QRRAHVSRRM GFLMHVLERE QMERVRAARP LPEFRAGDVL EVRMMVPEAE RKVVVYRGVC IA VYKKGIR SSFKIYNVFP DSGGVVQHIP LYMPDLVGVK VVGRIPASQE RLYYLLERET GTSDYTFQTN VSAAK

UniProtKB: Uncharacterized protein

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Macromolecule #12: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 13.140527 KDa
SequenceString:
MPINRQKILQ LASSFRGRAR TCINIARLRV EKALQHAYVG RKLKKRHWRS VWIQRINAAS REHGMSYTGL ITGLKAENVA LNRKVLSEL AMSEPFSFKA LVEQVRHMRG LPPAGP

UniProtKB: 50S ribosomal protein L20

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Macromolecule #13: bL21m

MacromoleculeName: bL21m / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 40.170922 KDa
SequenceString: MSLVRAALSQ LADSLSFGIL GVNRSCWGAS TSRATGLPSS ISGVRLLRTL AGTGDDQRAL PFNSRATVDE PFALPSTLAS STGCPTTPT PHAARLPALA ALHPPAGSLL PSLCQQLRGY KSNKRSLKPL NTAASWQAVQ PLLAEQRQRA AVEGVSPPVA D GARPPPQP ...String:
MSLVRAALSQ LADSLSFGIL GVNRSCWGAS TSRATGLPSS ISGVRLLRTL AGTGDDQRAL PFNSRATVDE PFALPSTLAS STGCPTTPT PHAARLPALA ALHPPAGSLL PSLCQQLRGY KSNKRSLKPL NTAASWQAVQ PLLAEQRQRA AVEGVSPPVA D GARPPPQP GRRRWRPRLG PIVDTCLPPI PAVPLPTECR RVGVITGQYS LEPERTFAVV ELAGSQYKVT TDDVLFVNQL KG VAVNDVV ALERVLLLGS RAETVVGRPY VPGGVVLAAV EEHFRDGKVH VFKKRRRKRY RKYQAPRPHL TTLRILQVRG IDP APGDES ARLPELPVAL LDARYESVLR LTGRGEQQQQ QQQQQDEEQR QAAA

UniProtKB: Large ribosomal subunit protein bL21m

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Macromolecule #14: uL22m

MacromoleculeName: uL22m / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 39.18007 KDa
SequenceString: MSGAETHRLA LLASSRALIA RVQLGDAPAA LSQALSTLSS VIYASTSYGR SECSCSSSSG REVQPSATPA SPATAQTSRV APFLALQRP SAAFSRLGLS TWAPHASVSG WHGARQLHAG SAAQQAADVP GGSASGSGGK ESEGAAKPQE QTVTNPLAAA L ASAASGPV ...String:
MSGAETHRLA LLASSRALIA RVQLGDAPAA LSQALSTLSS VIYASTSYGR SECSCSSSSG REVQPSATPA SPATAQTSRV APFLALQRP SAAFSRLGLS TWAPHASVSG WHGARQLHAG SAAQQAADVP GGSASGSGGK ESEGAAKPQE QTVTNPLAAA L ASAASGPV ASGSASAAGG LAQAAQAAAG RGRRQPRNWM WYDADDEREE RRKERQRLAW AMGPGGGEEV GAAHLMDVPQ SM KKMQRIV KLVRGLPYPD AVAQCSLVPH KAARYMLQAL EAAHADATEV KGLDAERLVV GTVFVTRGAY EPGISYHSKG RPG SKTFHR SHIRVVLNQA AERPAPFARI VAPLMSRRGL LGGSGGAGGA PRPRFAYRTE V

UniProtKB: Large ribosomal subunit protein uL22m

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Macromolecule #15: Mitochondrial ribosomal protein L23

MacromoleculeName: Mitochondrial ribosomal protein L23 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 25.593826 KDa
SequenceString: MRWQHSAAEQ TASTRAIKGS ADDTPSAAPE QETRLRFVSS EWRSGRPQPP ERRIPICFPN ITLQMMKLTP EQMDQVKETG WLREVAFKT TPDVTKLEIA AFLQSVYGMN VERVSTINYL GRRRLAISRN GKRLWWREDD WKKAYVIFRP PAGQEHLLTR G RRAGGDGD ...String:
MRWQHSAAEQ TASTRAIKGS ADDTPSAAPE QETRLRFVSS EWRSGRPQPP ERRIPICFPN ITLQMMKLTP EQMDQVKETG WLREVAFKT TPDVTKLEIA AFLQSVYGMN VERVSTINYL GRRRLAISRN GKRLWWREDD WKKAYVIFRP PAGQEHLLTR G RRAGGDGD SGDEEQQRPV IEQIREAARA PRLPPRPYPW ERRPGGGRRG APGGEAEVPG GRSGDAAE

UniProtKB: Mitochondrial ribosomal protein L23

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Macromolecule #16: KOW domain-containing protein,uL24m

MacromoleculeName: KOW domain-containing protein,uL24m / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 31.1646 KDa
SequenceString: MPQALYRSIQ PMFKTNRWRI CRDDLVYILS GPDKGKTGKV LEIIKDTRTP QVIVEGRNLR KKKVKTGTGP DDFFVVTMEA PLHYSQVQL VDPQTGKPMR VIFAYTADGT KVRSRKVPNP STADIIATPS EEDPDPRVGL TGPKDTSASL ARQATYTPSA G FPFRVRNL ...String:
MPQALYRSIQ PMFKTNRWRI CRDDLVYILS GPDKGKTGKV LEIIKDTRTP QVIVEGRNLR KKKVKTGTGP DDFFVVTMEA PLHYSQVQL VDPQTGKPMR VIFAYTADGT KVRSRKVPNP STADIIATPS EEDPDPRVGL TGPKDTSASL ARQATYTPSA G FPFRVRNL ERIWAELQPD AD(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

UniProtKB: KOW domain-containing protein

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Macromolecule #17: Ribosomal_TL5_C domain-containing protein

MacromoleculeName: Ribosomal_TL5_C domain-containing protein / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 38.730926 KDa
SequenceString: MRCATGATAS LLPAARSWAV SMLTSGLDHG LGVSVLGGGP LPGLLARARH DSALAQRASE QVVPPEILRE LQQLEGSLND AARLDGRRR SSLRLSQSTL PLPLPTHTVE PGLPALDAHV RPRSATGGTA CKWLRRAGRV PGRVHSLPGA DARAGAAAGH S EDQGASSS ...String:
MRCATGATAS LLPAARSWAV SMLTSGLDHG LGVSVLGGGP LPGLLARARH DSALAQRASE QVVPPEILRE LQQLEGSLND AARLDGRRR SSLRLSQSTL PLPLPTHTVE PGLPALDAHV RPRSATGGTA CKWLRRAGRV PGRVHSLPGA DARAGAAAGH S EDQGASSS SSNINNNNNK LLVHFSEGDV AKLIRTFGRN GCTARVLQLN LVAPDAAASD SGAEQAAQAD AGRSGEGTSG ST SGTQLGM LRVKPTRVFV NAVTQRVEGL DLIYCPPERQ VLVDVPVRLL NDDLAPGVKK GGWIALFKRT VRYKALGGAI PPY IEVNVR NMDLDQDMLV RDMPIPPGTK LYEKDYNAPV LRCTTDVGKD

UniProtKB: Large ribosomal subunit protein bL25 beta domain-containing protein

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Macromolecule #18: bL27m

MacromoleculeName: bL27m / type: protein_or_peptide / ID: 18
Details: N-ter is extend compared to A0A2K3E880,N-ter is extend compared to A0A2K3E880,N-ter is extend compared to A0A2K3E880,N-ter is extend compared to A0A2K3E880
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 18.010873 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)MKLT AGELAFPGMI ILRQRGTKFH PGANVGIGRD HTIFATGVGK VRVDTQ PGP RGERRVVSVE PLPEALAAAS SPATPGAAAD ADSALRLRQV EAELVKRRAE VKRAMLQGRT PLEPALYFPL PRTADGQ LS WLERSVAKAP ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)MKLT AGELAFPGMI ILRQRGTKFH PGANVGIGRD HTIFATGVGK VRVDTQ PGP RGERRVVSVE PLPEALAAAS SPATPGAAAD ADSALRLRQV EAELVKRRAE VKRAMLQGRT PLEPALYFPL PRTADGQ LS WLERSVAKAP AVTPSAAAGK DAVPAKPKAA AKKAS

UniProtKB: Ribosomal protein L27

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Macromolecule #19: bL28m

MacromoleculeName: bL28m / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 17.000568 KDa
SequenceString:
MSSNVRQLIS RLIPPARKQF EHLQAHRRDV VWGDTQITLR VRQYPKSKDE RVSLVLPNWH RVRLWSEALG RKVELVMTKD TLRHIEDMG GLDAYLIKTP ESRLKSNPAS AVKWEVMSAL RRREAAAMMA RGGRGGAAGG DGSGAAGAAG KEST

UniProtKB: Mitochondrial ribosomal protein L28

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Macromolecule #20: uL29m

MacromoleculeName: uL29m / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 14.799233 KDa
SequenceString:
MRFDVENQGG LACVPKPCVS STGRGWKVEE LRLKSWQDLH KLWYVSLKER NLLLTEMGLK HIPKDAAHQV MRRIPVGAAS VEEDPHRLR YTEVQNTLRH IRQVLEERAA AELVPKLRRE MLEVINAR

UniProtKB: Predicted protein

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Macromolecule #21: Ribosomal_L30 domain-containing protein

MacromoleculeName: Ribosomal_L30 domain-containing protein / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 24.367816 KDa
SequenceString: MATEGSVQAV KSLFVTLRRG FAGTPWFHRR VLESLGLKYR HQCVEKPNNS SIRGMLSKVP HLVVIETDRM RYLRELKEHY QQLPREPWV LHHDPPLPAA TPSNPSSPLA STSSSDSDST ELLTPAPLPP SVPAQPRLTP FAASQLREHV EPVGLEGYRA N KQPPLPPR ...String:
MATEGSVQAV KSLFVTLRRG FAGTPWFHRR VLESLGLKYR HQCVEKPNNS SIRGMLSKVP HLVVIETDRM RYLRELKEHY QQLPREPWV LHHDPPLPAA TPSNPSSPLA STSSSDSDST ELLTPAPLPP SVPAQPRLTP FAASQLREHV EPVGLEGYRA N KQPPLPPR KYFQEQSRRW RAKVLTERGV FSADACREHA EAIRPKSYRR RTGIRD

UniProtKB: Large ribosomal subunit protein uL30m

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Macromolecule #22: bL32m

MacromoleculeName: bL32m / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 13.370118 KDa
SequenceString:
MDSTSHPSTS YVVAPRLPAE EDDTFVPFSR PSPSPFGSGN SLLPQLCATP KKKLSLHRRG NRRVWYWLKR KTEVAKCSYC GALSGRNQM FSGAAGNSAC TGQCVEQAPT PPASSYPAEY KPPC

UniProtKB: Uncharacterized protein

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Macromolecule #23: Mitochondrial ribosomal protein L33

MacromoleculeName: Mitochondrial ribosomal protein L33 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 6.864234 KDa
SequenceString:
MSNGGKKGVR LLVKLVSTAK TGFFYVTEKN PRNTPWKLKL MKFDPKVNKH VLFEEQKLK

UniProtKB: Uncharacterized protein

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Macromolecule #24: bL34m

MacromoleculeName: bL34m / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 29.646521 KDa
SequenceString: MASSSSVIAR AGQYGSLLLQ GPALSQATSN LGSLFSSTQA PPSADLSFSE RSSFALERLD TLTASSSGRQ HASGDAGLFV GFGGGAGGP LTAAHDSGLA PTICLWDSRL VSFDVSRLLA EAHRGRHQPQ TLSAQQPQLS HQELLGAHQH LPRLQMHLGG A DGVTPRLQ ...String:
MASSSSVIAR AGQYGSLLLQ GPALSQATSN LGSLFSSTQA PPSADLSFSE RSSFALERLD TLTASSSGRQ HASGDAGLFV GFGGGAGGP LTAAHDSGLA PTICLWDSRL VSFDVSRLLA EAHRGRHQPQ TLSAQQPQLS HQELLGAHQH LPRLQMHLGG A DGVTPRLQ LHAPLTPPEL WAPHVLPGAP RPLQPHQPHQ PPAPAGLPAT PAPAPAPAPP APGGPAPAPL RCGNRGNTYQ PS RRKRVNR HGLEKRLSTP QGREVLLRRL RKGRWRITVD SFR

UniProtKB: Large ribosomal subunit protein bL34m

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Macromolecule #25: bL35m

MacromoleculeName: bL35m / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 17.493998 KDa
SequenceString:
MPWSLAHTPR GLFGAARPLV NPQIHAHDKY KKENPQPANS FLLGRFVTDR NGIIWHRQAN YRHARHAKSA SQLTRLKRWK PLAPAFAAK LRKLGFSERY WAAPDPQDVP GFHSPRGRVE RPRRSAVPDM DHTTGEPALR QSWQPPNRQR LR

UniProtKB: Uncharacterized protein

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Macromolecule #26: Plastid ribosomal protein L6

MacromoleculeName: Plastid ribosomal protein L6 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 22.275268 KDa
SequenceString: MLTTRVKPFR AAAVSRSSRL CVVAKDSRIG RAPITVPKGV TVTLEGQLVR VKGPNGTLEQ TLSPLVKIEQ ADGKLKLFKL ADDRVAMSQ HGLNRSLVNN LVVGVSTGFE KRMEMVGTGY RAAVAGKDLT LNVGYSKPRV LAIPEGLKVV VEKNTTLVIS G ADKVKVGD ...String:
MLTTRVKPFR AAAVSRSSRL CVVAKDSRIG RAPITVPKGV TVTLEGQLVR VKGPNGTLEQ TLSPLVKIEQ ADGKLKLFKL ADDRVAMSQ HGLNRSLVNN LVVGVSTGFE KRMEMVGTGY RAAVAGKDLT LNVGYSKPRV LAIPEGLKVV VEKNTTLVIS G ADKVKVGD FCATIRRQRP PEPYKGKGIR YAGEVIKLKE GKGAGGKKK

UniProtKB: Large ribosomal subunit protein uL6 alpha-beta domain-containing protein

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Macromolecule #27: mL40

MacromoleculeName: mL40 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 17.749047 KDa
SequenceString:
MAGFTKATST KGAAMKRNKG AASGGVDEEN ARVKAMVAML QPPAPAEATP LTPAEQAAAQ QLQQQRVAEH QAWVKDMTTK FKLQQAALR ALPERLRVLA MQPDHTPYPL NRKFLFDSPP AAYRDTPAGK TASGASAAAE ASAGAGKGAE AASGGGKQAG G KQGGSRKQ QGGR

UniProtKB: Uncharacterized protein

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Macromolecule #28: mL41

MacromoleculeName: mL41 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 12.623615 KDa
SequenceString:
MTVRSIVVSL IRGANKASRQ HQGDIGREAV VDLIQQSAAK QSGIRKGWQV KAATWVKRVH VDRGDVKVGR LEGGEFQVLP HLRPRYFVP ADLDKFQLKP YVEVEKKVEA AKQ

UniProtKB: Uncharacterized protein

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Macromolecule #29: mL43

MacromoleculeName: mL43 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 16.217593 KDa
SequenceString:
MSKYGVWMLE SLVIKYCDIG GSSRGMRLFL DEALPALRQQ NPQLGVQQVL QRFRHPKLVA VYRNGRTKPV CVKNLAPAEI MEHIGWLRN SHGRGQEYQV VRSRHLSRSP SIQGTWSVDT FASQLERVNE ARRAREAAQT QY

UniProtKB: Predicted protein

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Macromolecule #30: Mitochondrial ribosomal protein L17

MacromoleculeName: Mitochondrial ribosomal protein L17 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 27.320754 KDa
SequenceString: MRSAGSLIAK SLFAVSTCGN RSCAGLLTRG YGAEAASATV NDGASTSGEG PIIAACVLQR LPLVRPRLTT GEQAQLALER QRALQTGEL KDYPQNMAVT GKDDAGSKQQ QQDLRAFDPV PATTAADSNG DVSTMRRRLA DSLYLVVRGG PAAGGGGGEW A FPQAVNTA ...String:
MRSAGSLIAK SLFAVSTCGN RSCAGLLTRG YGAEAASATV NDGASTSGEG PIIAACVLQR LPLVRPRLTT GEQAQLALER QRALQTGEL KDYPQNMAVT GKDDAGSKQQ QQDLRAFDPV PATTAADSNG DVSTMRRRLA DSLYLVVRGG PAAGGGGGEW A FPQAVNTA EESISDTAQR ALYGAIGRSH PVYFIGNAPM GHVRALPAGT SFFMLAQVVD DPWDTQLLPG AAQELAWVTK QE LLGSYLS DARLRELVSK ML

UniProtKB: Uncharacterized protein

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Macromolecule #31: mL63/57/60

MacromoleculeName: mL63/57/60 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 14.645161 KDa
SequenceString:
MFFSRCVMVV FKTTGGRSWN PPSGLRPLSP AQRRNRTKNL ALTMKNMSIL KLAEANQPEV PVRLYKPLNF SRMQWMKKKL EETRAALGW DMEARALQEQ ARALRVGGGR QGAAGSLLPP AARAALQGSV GDK

UniProtKB: Uncharacterized protein

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Macromolecule #32: mL59/64

MacromoleculeName: mL59/64 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 14.372869 KDa
SequenceString: MAHLLARLGA KALLPRRDGD KLLPPLIGAA EALRLREQFY AIGQQWPYEN IVPGKPPSPP GCKAYLERQK AKEAKRAARA KQVAEAMAN MPKTVADYKA SRRLDWSEVS ALDR(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...String:
MAHLLARLGA KALLPRRDGD KLLPPLIGAA EALRLREQFY AIGQQWPYEN IVPGKPPSPP GCKAYLERQK AKEAKRAARA KQVAEAMAN MPKTVADYKA SRRLDWSEVS ALDR(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

UniProtKB: Predicted protein

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Macromolecule #33: mL80

MacromoleculeName: mL80 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 27.176438 KDa
SequenceString: MHASVSGSLD TAPSSSSGTA LATASTSSAP LDLREQRHLY LDGTRTADPG EPRYTAPYWV PPSARAGIPN ILFSEPWPSH EEPQLRRQH AAMCLEALKR ADRPLTAEQV HEAVNSTAGY SASASAGDAG DSGAGADKPV LSTLAYTKKL LEHLRRTRFV Y GRKNPDSM ...String:
MHASVSGSLD TAPSSSSGTA LATASTSSAP LDLREQRHLY LDGTRTADPG EPRYTAPYWV PPSARAGIPN ILFSEPWPSH EEPQLRRQH AAMCLEALKR ADRPLTAEQV HEAVNSTAGY SASASAGDAG DSGAGADKPV LSTLAYTKKL LEHLRRTRFV Y GRKNPDSM LSPGHPDHPR LYEALPFQAA RYGKPETLAA ADEAARAAAI AKAQKRLRNG KAPYPQHRRR ARFSIWQHEL AQ EALRELQ AK

UniProtKB: Uncharacterized protein

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Macromolecule #34: mL87

MacromoleculeName: mL87 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 23.535336 KDa
SequenceString: MLALLAVRAR SPSLPSITLP ARLLSTQTSA SVSETYSNRP TSSAESTEAV SSSGQSASKW DWKWVLGKAS GRKPAITRPR RHQWHYCNP EYDPAAPLPE VLRSPFGPPG AERSHDWATY ARHLQLQPEN RRDLKRYRAR FVRFMQLREL DWREAFQRGV A EDSRVSNK ...String:
MLALLAVRAR SPSLPSITLP ARLLSTQTSA SVSETYSNRP TSSAESTEAV SSSGQSASKW DWKWVLGKAS GRKPAITRPR RHQWHYCNP EYDPAAPLPE VLRSPFGPPG AERSHDWATY ARHLQLQPEN RRDLKRYRAR FVRFMQLREL DWREAFQRGV A EDSRVSNK VARAKAEAQR QDAWSDYKQA MWQRAQLADS HQSHGTGR

UniProtKB: Uncharacterized protein

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Macromolecule #35: bL36m

MacromoleculeName: bL36m / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 3.08179 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

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Macromolecule #36: mL113

MacromoleculeName: mL113 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 84.437922 KDa
SequenceString: MRQLSLSLLA RLGRGSRGSL QPASSAINSG VDPGVLSGEE CSTSAPAGMP SWLRHSRRYA HQYNLIQPVD TNHINALLSS ATALEHAAV PVLRYSAWFD PEQVTRTMQR VPRMLQYQRR KGRRGAAYAS SPSSSADLAR SLLDALGSRL AALAPACSDQ Q LARALWAL ...String:
MRQLSLSLLA RLGRGSRGSL QPASSAINSG VDPGVLSGEE CSTSAPAGMP SWLRHSRRYA HQYNLIQPVD TNHINALLSS ATALEHAAV PVLRYSAWFD PEQVTRTMQR VPRMLQYQRR KGRRGAAYAS SPSSSADLAR SLLDALGSRL AALAPACSDQ Q LARALWAL GAARHPHPQA LAAACEVLPQ RLKGASGAAA AAGAGSGAGG MAMTDLATAA WGLAAAASAG PQSVREPVRR AL QEVARHL VASRPADLSA TPALPQPSSP SSISSPSSGA VAPAADEVAA AASAAALAAD RPWLDPRSAV KLAWAFASCE VKD AAALDV VAEAAEARIA SQLQAHDPTT GPLTPRATYM YQTIRGWQAW PRPRPRVIRS AASAARGGRS RYLYDDRPRV VLRD FTAGS LAQLLAALAA AGHRHEGLMQ AAAAHLTASS GRSLRVDPHD LKRLAAAFAR LDLAAPAAAS GGAATAAALT ALLSA AQLS SLPAPLLARL AILAAESGVR RRSVYDRLVR QLMARAWVP(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

UniProtKB: Uncharacterized protein

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Macromolecule #37: mL114

MacromoleculeName: mL114 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 48.757883 KDa
SequenceString: MRRAVARCTA QIAAEACSVS SACSTSGRQV QEQLDNCLRW MTYARPRSRI DYYDPTRNVY KEFQRKWDRA NAAAAAAASQ AEAAAARPS HSAPPSAAPQ QQPYGGAGSY PFGSSLTTPC SSYGSGYGAG YGGTAAAGAD WPPGYEALLQ TYLEQELPLS A EEASTLVA ...String:
MRRAVARCTA QIAAEACSVS SACSTSGRQV QEQLDNCLRW MTYARPRSRI DYYDPTRNVY KEFQRKWDRA NAAAAAAASQ AEAAAARPS HSAPPSAAPQ QQPYGGAGSY PFGSSLTTPC SSYGSGYGAG YGGTAAAGAD WPPGYEALLQ TYLEQELPLS A EEASTLVA AAKKGLLPGS RSLIRTRFMH IKDLEPRFPG FDARAAVLGE PRLLRHAADK VMRAMLVFQD HWPSHPVGPL MG RIGCPVI RDPAGVGHRL YALTRALKTD LHYELDPHRL TPESEGFLAS GVSPFELEAR VSALVTIFGR EGAGRLLDVS LDV LTYAPR DLDRAVLALR EVFSAAGDRG YGRHSLSTPE GAAAAAADRG YVTDLAVAWP GVLALPGRLG GADGVARLLA RVRR AGGAR YRGAVGRRAL LSEVLERPEL LQAAAEAAMR GEEEDEEEDV EGRAELEGKV

UniProtKB: Uncharacterized protein

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Macromolecule #38: mL115

MacromoleculeName: mL115 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 72.344484 KDa
SequenceString: MSIALLCRRQ GRGLARALSL IESGASIERD VAITDENIRG SDAGACSTSR QCQPDTPIGG FRQWPRSLQL WRGFAVNNKP SLKYVPVPE SQHDDFTSEP TTYTQLLASI RAAKSLKRLE HLVDTYADRF DAVHVAAAVA RLPHLLKYRE ADLVDMSASA V VLPSGMTR ...String:
MSIALLCRRQ GRGLARALSL IESGASIERD VAITDENIRG SDAGACSTSR QCQPDTPIGG FRQWPRSLQL WRGFAVNNKP SLKYVPVPE SQHDDFTSEP TTYTQLLASI RAAKSLKRLE HLVDTYADRF DAVHVAAAVA RLPHLLKYRE ADLVDMSASA V VLPSGMTR TRRKHGAQLR SGSAEVAARL AARVDAMLPQ HVAHFFPRQA ACSIWAFGEL RRHGVIERMD SLPQVLMSVT RG NLQPLRV HAAGVDFAQL LHGLAKLGHN DEPLLDALLP LVTERLGSMQ QRELQMTVWA LATLRRATPE LLDEVAQQLL STS TAFLLP SACASVFWSY AKTEGLARLS PGGEGGARVS RRRARVAAAR VKLFDSLAAT MMAQALLLAP QDVATTLWAC SVLG YHHSQ LPAVLGDAVL RALPNCSDAE VASVLESLAH LGYHHAPLMD AVAAGILAEP VVSTEPVNIA RVLYAYGVLA RRGPR DLQL VGTLAEALVR RLARVERLDT VALACRGLGA FAYDDQAVLA QVAARTEQLL QTSTTGLEQL QAVLRCLDAG GCSYFQ LAV AAARMLDDRL RAGACRSAPL AVEVLYYSAR QGV(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)

UniProtKB: Uncharacterized protein

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Macromolecule #39: mL116

MacromoleculeName: mL116 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 53.018789 KDa
SequenceString: MRQQAGMLLG EAVASTSGRA SPALQLIIQR TLSLVASGIP SPRADVALQL STPHGGHINR MINTSESIVE LDSILYRFRK RLRPANIGA AAMRLEHLNR LERRTPYALR VQRVAAELQK YVATYTDRLA LTQAANVLRG LSAVRHRLPP ELVLRLAAGA V ADGGAALR ...String:
MRQQAGMLLG EAVASTSGRA SPALQLIIQR TLSLVASGIP SPRADVALQL STPHGGHINR MINTSESIVE LDSILYRFRK RLRPANIGA AAMRLEHLNR LERRTPYALR VQRVAAELQK YVATYTDRLA LTQAANVLRG LSAVRHRLPP ELVLRLAAGA V ADGGAALR LAPDVDVRDL CFGLAGQGFN NTAFWARLCA AVLPRLRSFD PNTLPALVTA LQAAQQLPAP ASASASSGSA GS AVAAAAG GSTPQAAVAA EALRLLSRSE TLAALAPARL ADAASLLAGL GPALGVAVDA RLVEAVQTAT ARALPSLSPN QLP GLLLAV AALRRAAAPA EAAAAAAATA PQQQLPAALL ATALPHLSAG AVTMDLTAVM RAARLLAPHA AEPAAADTLV RLAR RTLLL LPAPGSSTGG PTGSASGSSS SNGEGLVTLS RVPRGGQAAG AVLAAAAPAG QLQGRTAGAV EGVARAFAAA APAVA PQPA LVGELAARLA AAGEAAAARG LLDEAQLASL GRSVEVLAAA GAAKSG

UniProtKB: Uncharacterized protein

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Macromolecule #40: mL117

MacromoleculeName: mL117 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 75.804086 KDa
SequenceString: MLQALAGGAL GGLQTNGPAN LVGALGLLQR AAAAVVTGVP SSSSPVPPHA DRSLASLSAG AQSAAESACS HGGCGHDEAP CCSARSSSN SSSDAGAPRG LQQQLRSQQL QQHQHQQRRG IATSAGSALA YKFQSNVSPA SSRGSGRGSK VATRDNYQRW R ESGGDVRV ...String:
MLQALAGGAL GGLQTNGPAN LVGALGLLQR AAAAVVTGVP SSSSPVPPHA DRSLASLSAG AQSAAESACS HGGCGHDEAP CCSARSSSN SSSDAGAPRG LQQQLRSQQL QQHQHQQRRG IATSAGSALA YKFQSNVSPA SSRGSGRGSK VATRDNYQRW R ESGGDVRV AQDILREAEG SGRGGGGGGG AGAARRGDSR LRRGADRPGG SGAGSGGGTG AGAGVVDVQD ELRAMVLGCR DL SELQVVV CECGADLNPF LVCAAAARLH KLKQATPPGA SPAALARRVG ESLMVLLQDR AAEAPLSQLA GAAHGLAEAG LAP GAALLE ALAARCEAAS PRGG(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) AAASELGAG MLRPLCDALT PRVPALSCAD VASLATGLAA ALGAAGGEAA AAAADGAPPL LSPSHFGSLP RLLSDLLLLR G PGQFGGRN FASVALALAL VTGGPAGAGG GGAAAAGSLP PAFWSKLAAV ALPEVPAMDA GSLSRLAGAF C(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

UniProtKB: Uncharacterized protein, Uncharacterized protein

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Macromolecule #41: mL118

MacromoleculeName: mL118 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 64.122902 KDa
SequenceString: MAFLVAARRQ LKGGLSLAEV ALPVSVLGTC TRSNAVFTEA LAGCSGASLH CSAEQPLSRA TSYSDAGAGA AGVPACGSAS PRQPSPEPC SSSGSHATFN SLGRYRSQHV GISRGASALS ALAEASASPS PASNLPRGQH QHQQHHHQQL RTYHAWYYGS K LRNRAISQ ...String:
MAFLVAARRQ LKGGLSLAEV ALPVSVLGTC TRSNAVFTEA LAGCSGASLH CSAEQPLSRA TSYSDAGAGA AGVPACGSAS PRQPSPEPC SSSGSHATFN SLGRYRSQHV GISRGASALS ALAEASASPS PASNLPRGQH QHQQHHHQQL RTYHAWYYGS K LRNRAISQ AESLEELGEM LVREGHRLDH VNLTALLAQL KRVARAAEEE AVAEATGGSS SSSSNSSSSG SSAVTAAAAA AA ARAVRVR VAELAAVAAR LVRRRAKWYD PRHAALAVAH TAALRHTDGR LLHDMTGRAL ARLDEAYSRD VLLLLRGLCA HQH MQQLAA ASSPPAVAAA VAPAVPAVAG AGKPYGGAPA VLLGGVKVFL TAKVPTGRMP PENLAGLLRH WRALAPPGRR LGPA VCGVV AADLQTRTAI YAPEPLAGVL ATLSAERHAL PPPLLDAAAE QFAAHALTHG SGAAAARFLA AVGAQLRLQQ QA (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

UniProtKB: Uncharacterized protein

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Macromolecule #42: mL119

MacromoleculeName: mL119 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 36.808164 KDa
SequenceString: MATGAVAASA ADTASTSAPA PPSSSPFMPR WLRNLFPGGE HLPASPAMER QTSGASASSS GGSGPGSEAD DIKQLEEMRN MDMQGYVEY CKKMRGGAPP PRPRRPSVSP DHYDYRTMQD QRRIAFLRMQ QHEHIGSLVT KEESDLILAK REDVVKNRAL L QAIADRTG ...String:
MATGAVAASA ADTASTSAPA PPSSSPFMPR WLRNLFPGGE HLPASPAMER QTSGASASSS GGSGPGSEAD DIKQLEEMRN MDMQGYVEY CKKMRGGAPP PRPRRPSVSP DHYDYRTMQD QRRIAFLRMQ QHEHIGSLVT KEESDLILAK REDVVKNRAL L QAIADRTG VYIDLEVKDC IEQFLETREN AGQMHRYATE FGMPLPKGSQ EQREMRRFMK RVEAEEKLAV ALEKRDLTSC SL RHKLSWA GPTALCDQTT LRYHECC(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)K REVVDKDRER QVHGMFKSRA EGILRKAAMD GVKPRLRDY

UniProtKB: Flagellar associated protein, Flagellar associated protein

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Macromolecule #43: Unk1

MacromoleculeName: Unk1 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 2.656265 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

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Macromolecule #44: PPR*

MacromoleculeName: PPR* / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 14.656045 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

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Macromolecule #45: Unk2

MacromoleculeName: Unk2 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 4.188154 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

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Macromolecule #46: L1 rRNA

MacromoleculeName: L1 rRNA / type: rna / ID: 46 / Number of copies: 1
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 51.976762 KDa
SequenceString:
GGGAUUGCUG ACUGAUAGUG CAAACAGUAC UGUGAAGGAA CUAAUGAAAC UGGGCUAUAA GUGCGCAUUC CACCAAUACG UGAGCAUUC GCGCAUUACC AUUCUCCAGA GGAUUUAAAU GCACAAACCU AUAUGCCUUU UGGCAUAUGA UUACACUUCG U UA

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Macromolecule #47: L2a rRNA

MacromoleculeName: L2a rRNA / type: rna / ID: 47 / Number of copies: 1
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 27.512189 KDa
SequenceString:
CAGCUAUAAU UUAGCGUACC UUUUGCAUUA UGGGUUAGCU ACUCAAUAGA CGUGAGUUAA GCAGAAAGCU UACUGAGAUU UUUUUA

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Macromolecule #48: L3a rRNA (5S)

MacromoleculeName: L3a rRNA (5S) / type: rna / ID: 48 / Number of copies: 1
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 24.033252 KDa
SequenceString:
GGUGCUCGGU GAAACCGAGC AUCCAAUACU AAAGAAACUU UACUGGCUUA GUACUGGGAC CCCAUUUUUA GUAUA

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Macromolecule #49: L3b rRNA

MacromoleculeName: L3b rRNA / type: rna / ID: 49 / Number of copies: 1
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 59.395223 KDa
SequenceString:
ACGUCUAUUG GACCCGAAAC CGAUCGAUCU AGCGACGUGC AGGUCUGACU UUAAUCAGGA CCGAACCCGU GUCUGUUGCA AGAGACUGG GAUGACACGU GGCUAGGGGU GAAAGGCCAA UCAAGUUCGG UAAUAGCUGG UUUUCUGCGA AAUCUAUUCA G GUAAAGAG UGUGUACUAA GACCAU

+
Macromolecule #50: L4 rRNA

MacromoleculeName: L4 rRNA / type: rna / ID: 50 / Number of copies: 1
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 38.253898 KDa
SequenceString:
AUACCGGUAG CGCAAGCGAA UGGUAAUGGA UCUAAUACAA CACAUUCAGA CAAUCGGCGG UAAGGUCAAU UGUCAAGAGG AGAACAGUC CAGAGGCGAG GCUAAGGUGC CUAACAAUU

+
Macromolecule #51: L5 rRNA

MacromoleculeName: L5 rRNA / type: rna / ID: 51 / Number of copies: 1
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 47.720117 KDa
SequenceString:
AAGGUAGAGU CUGCGUUGAA CACCAGAACA UAGGCUUGGA AGCAGCCACU GUGCUUGGAA AGCGUAAUAG CUCACUGGUC UAGCAGUCU CCGCUUAUAU AUGUCCGGCA CUAUUGCCGA AGCCCGCCUA UGACAAAUUU UUUUUUUUUU

+
Macromolecule #52: L6 rRNA

MacromoleculeName: L6 rRNA / type: rna / ID: 52 / Number of copies: 1
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 43.923266 KDa
SequenceString:
AGUCAUAGGU AGCAGAACGU UCUGGAACAA UUACGCUGAA AACAGUAACA GAAUUGAAGC UGCUAACAUA AGUAACACUA AAUGGAUAA CAUGGCCGUA AGCCCAAGGU UUUGCAUAAC AAACAGUGAA UACGGUU

+
Macromolecule #53: L7 rRNA

MacromoleculeName: L7 rRNA / type: rna / ID: 53 / Number of copies: 1
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 186.482641 KDa
SequenceString: UACCGUACUG CAAACCGACU CAGGUGGGCU AGUAUAUAUA CUCAGGCCAU AGAAUCAACC AUGUUAAAGG AACUCGGCAA AAUGCACGC GUGACCUCGG AGGAAGCGUG GCUAAAAAGA CUCAGCAAAU AGCCGACAGC GACUGUUUAC CAAAAACACA G GAUAGUGC ...String:
UACCGUACUG CAAACCGACU CAGGUGGGCU AGUAUAUAUA CUCAGGCCAU AGAAUCAACC AUGUUAAAGG AACUCGGCAA AAUGCACGC GUGACCUCGG AGGAAGCGUG GCUAAAAAGA CUCAGCAAAU AGCCGACAGC GACUGUUUAC CAAAAACACA G GAUAGUGC UAACAGACGU AAAUUAAGCU GCAAAAAGCG GUUUUAUUUU UGGAUGUAUA CUAUCCGACG CCAAACGGCG GC AGUAACU CUAACUGUCC UAAGGUAGCG AAAUUCCUUG ACUUGUAAUU GGCGUCCUGC AUGAAUGGCG GAACGACUGU CGG GCUGUC UCUAACAUGG AAUCUGUGAA UUUGAAAUCU UCGUGCAGAU GCGAAGGCGC UGGCAAGGGA CGAAGAGACC CUAU GCAGC UUCGCUGGGU GCUAAGAGUU ACUCUUAUCC UCAAGCUUUU CUGGGACGGA UGCUUCCUAA AUAGUAACGG GAGUG AUCA AUGGUACUGC UACUUAAAUA GAAAGCAGUG CUUGACUGUA AGAACCACAG UUCGAGCAGG GACGCAAGUC GGCAUU AAU GACCCAGGGU AACAU

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Macromolecule #54: L8 rRNA

MacromoleculeName: L8 rRNA / type: rna / ID: 54 / Number of copies: 1
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 132.907203 KDa
SequenceString: GUUACCCUGA UCAACGGACA AAAGCUACGC UAGGGAUAAC AGGCUUAUCC GCCUUUAGAG UUCCUAUCGA UGGGCGGGUU UGGCACCUC GAUGUCGGAU CAUCGCGUCC UGUGGCUGAA GGAGGUCGCA AGGGUUGGAU UGUUCGUCCA UUAAAGUGGU A CGUGAUCU ...String:
GUUACCCUGA UCAACGGACA AAAGCUACGC UAGGGAUAAC AGGCUUAUCC GCCUUUAGAG UUCCUAUCGA UGGGCGGGUU UGGCACCUC GAUGUCGGAU CAUCGCGUCC UGUGGCUGAA GGAGGUCGCA AGGGUUGGAU UGUUCGUCCA UUAAAGUGGU A CGUGAUCU GAGUUCAGAC CGUCGUGAGA CAGUUCGGUU UCUAUCUUUG UCAGCGAAAG GCUUGUUUUG AAACGCCUAU GC ACAGUUA GCCGCUGGGG GCGAGUACGA AAGGACCAGA CCCCUAUAAA CCUCUGGUAC ACUUGUUGUC AUUUACACGG CAU AGCAAG AAAGCUAAGU UUUAAAAGGC UAGAUUGCCU ACAUUCAAUA ACACCGAAUG ACCCCGACUU ACAAAAAGUU UAGG ACUAA AUUUAUGA

+
Macromolecule #55: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 55 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #56: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 56 / Number of copies: 69 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #57: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 57 / Number of copies: 1 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1.5 mg/mL
BufferpH: 7.6
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 20 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Number grids imaged: 1 / Average electron dose: 45.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

+
Image processing

Startup modelType of model: NONE
Final reconstructionNumber classes used: 2 / Resolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 101291
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final 3D classificationNumber classes: 6 / Software - Name: RELION

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