7PGX

Structure of dark-adapted AsLOV2 wild type

これはPDB形式変換不可エントリーです。

7PGX の概要

• エントリーDOI: 10.2210/pdb7pgx/pdb
• 分子名称: NPH1-1, CALCIUM ION, CHLORIDE ION, ... (9 entities in total)
• 機能のキーワード: flavin, light-oxygen-voltage, optogenetics, photoreceptor, phototropin, signaling protein
• 由来する生物種: Avena sativa (Oat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18701.24

構造登録者

Gelfert, R.,Weyand, M.,Moeglich, A. (登録日: 2021-08-16, 公開日: 2022-05-11, 最終更新日: 2024-01-31)

主引用文献

Dietler, J.,Gelfert, R.,Kaiser, J.,Borin, V.,Renzl, C.,Pilsl, S.,Ranzani, A.T.,Garcia de Fuentes, A.,Gleichmann, T.,Diensthuber, R.P.,Weyand, M.,Mayer, G.,Schapiro, I.,Moglich, A.
Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine.
Nat Commun, 13:2618-2618, 2022

PubMed Abstract: In nature as in biotechnology, light-oxygen-voltage photoreceptors perceive blue light to elicit spatiotemporally defined cellular responses. Photon absorption drives thioadduct formation between a conserved cysteine and the flavin chromophore. An equally conserved, proximal glutamine processes the resultant flavin protonation into downstream hydrogen-bond rearrangements. Here, we report that this glutamine, long deemed essential, is generally dispensable. In its absence, several light-oxygen-voltage receptors invariably retained productive, if often attenuated, signaling responses. Structures of a light-oxygen-voltage paradigm at around 1 Å resolution revealed highly similar light-induced conformational changes, irrespective of whether the glutamine is present. Naturally occurring, glutamine-deficient light-oxygen-voltage receptors likely serve as bona fide photoreceptors, as we showcase for a diguanylate cyclase. We propose that without the glutamine, water molecules transiently approach the chromophore and thus propagate flavin protonation downstream. Signaling without glutamine appears intrinsic to light-oxygen-voltage receptors, which pertains to biotechnological applications and suggests evolutionary descendance from redox-active flavoproteins.

PubMed: 35552382
DOI: 10.1038/s41467-022-30252-4

実験手法

X-RAY DIFFRACTION (1.001 Å)