7PCX
X-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-Q165W LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATE
Summary for 7PCX
Entry DOI | 10.2210/pdb7pcx/pdb |
Related | 6ZVU 6ZVV 6ZVW 6ZVX 6ZVY 7PCW |
Descriptor | Haloalkane dehalogenase, [9-[2-carboxy-5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium, CHLORIDE ION, ... (5 entities in total) |
Functional Keywords | haloalkane dehalogenase, halo, tag, halotag7, self-labeling protein, fluorophore, tetramethylrhodamine, hydrolase |
Biological source | Rhodococcus sp. |
Total number of polymer chains | 6 |
Total formula weight | 204384.86 |
Authors | Tarnawski, M.,Frei, M.,Hiblot, J.,Johnsson, K. (deposition date: 2021-08-04, release date: 2021-12-15, Last modification date: 2024-01-31) |
Primary citation | Frei, M.S.,Tarnawski, M.,Roberti, M.J.,Koch, B.,Hiblot, J.,Johnsson, K. Engineered HaloTag variants for fluorescence lifetime multiplexing. Nat.Methods, 19:65-70, 2022 Cited by PubMed: 34916672DOI: 10.1038/s41592-021-01341-x PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.4 Å) |
Structure validation
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