7PCB

The PDZ domain of SNX27 fused with ANXA2

7PCB の概要

• エントリーDOI: 10.2210/pdb7pcb/pdb
• 分子名称: Sorting nexin-27,Annexin A2, CALCIUM ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: pdz, crystallization chaperone, peptide binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47507.12

構造登録者

Cousido-Siah, A.,Trave, G.,Gogl, G. (登録日: 2021-08-03, 公開日: 2022-04-20, 最終更新日: 2024-01-31)

主引用文献

Cousido-Siah, A.,Carneiro, L.,Kostmann, C.,Ecsedi, P.,Nyitray, L.,Trave, G.,Gogl, G.
A scalable strategy to solve structures of PDZ domains and their complexes.
Acta Crystallogr D Struct Biol, 78:509-516, 2022

PubMed Abstract: The human PDZome represents one of the largest globular domain families in the human proteome, with 266 instances. These globular domains typically interact with C-terminal peptide motifs found in thousands of human proteins. Despite previous efforts, not all PDZ domains have experimentally solved structures and most of their complexes remain to be solved. Here, a simple and cost-effective strategy is proposed for the crystallization of PDZ domains and their complexes. A human annexin A2 fusion tag was used as a crystallization chaperone and the structures of nine PDZ domains were solved, including five domains that had not yet been solved. Finally, these novel experimental structures were compared with AlphaFold predictions and it is speculated how predictions and experimental methods could cooperate in order to investigate the structural landscapes of entire domain families and interactomes.

PubMed: 35362473
DOI: 10.1107/S2059798322001784

実験手法

X-RAY DIFFRACTION (2 Å)