7PC5
The third PDZ domain of PDZD7 complexed with the PDZ-binding motif of EXOC4
Summary for 7PC5
| Entry DOI | 10.2210/pdb7pc5/pdb |
| Descriptor | PDZ domain-containing protein 7,Annexin A2, Exocyst complex component 4, CALCIUM ION, ... (5 entities in total) |
| Functional Keywords | pdz, complex, crystallization chaperone, peptide binding protein |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 2 |
| Total formula weight | 48788.92 |
| Authors | Cousido-Siah, A.,Trave, G.,Gogl, G. (deposition date: 2021-08-03, release date: 2022-04-20, Last modification date: 2024-01-31) |
| Primary citation | Cousido-Siah, A.,Carneiro, L.,Kostmann, C.,Ecsedi, P.,Nyitray, L.,Trave, G.,Gogl, G. A scalable strategy to solve structures of PDZ domains and their complexes. Acta Crystallogr D Struct Biol, 78:509-516, 2022 Cited by PubMed Abstract: The human PDZome represents one of the largest globular domain families in the human proteome, with 266 instances. These globular domains typically interact with C-terminal peptide motifs found in thousands of human proteins. Despite previous efforts, not all PDZ domains have experimentally solved structures and most of their complexes remain to be solved. Here, a simple and cost-effective strategy is proposed for the crystallization of PDZ domains and their complexes. A human annexin A2 fusion tag was used as a crystallization chaperone and the structures of nine PDZ domains were solved, including five domains that had not yet been solved. Finally, these novel experimental structures were compared with AlphaFold predictions and it is speculated how predictions and experimental methods could cooperate in order to investigate the structural landscapes of entire domain families and interactomes. PubMed: 35362473DOI: 10.1107/S2059798322001784 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.7 Å) |
Structure validation
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